The effect of Polysaccharides of Radix Paeoniae Alba on the syndrome characterization and endocrine/energy metabolism of liver Yin deficiency model rats
Objective Based on establishing a combined experimental model of liver Yin deficiency syndrome,to study the effect of Polysaccharides of Radix Paeoniae Alba(PRAP)on the charac-terization of liver Yin deficiency syndrome and endocrine energy metabolism in rats with chemical liver injury.Methods SPF male SD rats,weighing(200±20)g,were randomly divided into blank group,model group,Yiguanjian group,and PRAP group after one week of adaptive feeding.Except for the blank group,rats in all other groups were intraperitoneally injected with a concentration of 20%CCl4 olive oil solution and orally administered with 30 mg/kg thyroid tablets for 6 consecutive weeks.After 6 weeks,the levels of body weight,irritability degree,hair gloss,urine color,stool color and texture of the rats were observed and scored.Body weight,anal temperature,tongue surface humidity,food intake,water intake,serum cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP)content and cAMP/cGMP were measured.The contents of serum alanine aminotransferase(ALT),aliquot aminotransferase(AST),γ-glutamyl transpeptidase(γ-GT),alkaline phosphatase(ALP),total bilirubin(TBIL),and total bile acid(TBA)were measured.Liver was taken for HE pathology section examination.Serum testosterone(T),estradiol(E2)and crticosteroid(COR)levels were measured,and the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in the liver were detected.Results Compared with the model group,the PRAP group showed an increase in fecal score(P<0.01),while the other physical sign scores were significantly reduced(P<0.01);the body mass of rats in the PRAP group was increased,but the difference was not statistically significant(P>0.05).In the second and fourth week of the experiment,the anal temperature in the PRAP group was decreased(P<0.01);dryness and humidity of the tongue in the PRAP group was increased(P<0.01),but there was no statistically significant difference in 24-hour food intake and water consumption(P>0.05);the PRAP group showed a decrease in serum cAMP,an increase in cGMP,and a decrease in cAMP/cGMP(P<0.01);the levels of ALT,AST,γ-GT,ALP,and TBIL were decreased(P<0.01),while TBA content was decreased but had no statistical significance(P>0.05).Pathological staining showed moderate abnormalities in the hepatic tissue structure,with mild steatosis of hepatocyte in the field of vision.Numerous small vacuoles were visible in the cytoplasm,and some hepatocyte showed balloon like degeneration.There was a small amount of inflammatory cell infiltration in the liver parenchyma,and no obvious inflammatory cell infiltration was observed.The pathological state of the hepatic tissue in each medication group was restored.The serum E2 level was decreased,COR level was increased(P<0.05,P<0.01),and T level was increased in the PRAP group,but the difference was not statistically significant(P>0.05);the Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in the liver of the PRAP group were decreased(P<0.01).Conclusion PRAP can effectively protect against chemical liver injury caused by liver Yin deficiency,regulate cyclic nucleotides,endocrine disorders,and energy metabolism hyperactivity.This may be one of the mechanisms by which PRAP plays a protective role in liver injury caused by liver Yin deficiency,revealing the scientific connotation of nourishing liver Yin in PRA to a certain extent.
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万方数据
维普
