To explore the mechanism of Yulinzhu in improving ovarian oxidative stress in natural aging rats based on Sirt3-HIF1a pathway
Objective To investigate the effect of Yulinzhu on ovarian oxidative stress in aging rats and its mechanism based on Sirt3-HIF1a pathway.Methods Twelve female SD rats were randomly divided into aging without intragastric administration group and aging with intragastric administration group,with 6 rats in each group.Six female SD rats of normal reproductive age were used as the blank control group.The levels of serum hormone E2,FSH and AMH were measured by enzyme-linked immu-nosorbent assay.HE staining was used to observe ovarian morphological changes.The contents of superoxide dismutase(SOD),glutathion peroxidase(GSH-Px)and malondialdehyde(MDA)in ovarian tissue were measured by chemiluminescence.Western blot was used to detect the protein expression of Sirt3 and HIF1α in ovarian tissue.The expressions of Sirt3 and HIF1α mRNA in ovarian tissues were detected by PCR.Results Compared with the aging without intragastric administration group,the follicular development was improved in the aging with intragastric administration group,which was characterized by orderly arrangement of granulosa cell layers and regular oocyte morphology;the levels of E2 and AMH were increased,and the level of FSH was decreased(P<0.01);the levels of SOD and GSH in ovarian tissue were increased,and the level of MDA was decreased(P<0.01);the level of ATP expression was increased(P<0.05);the expression of Sirt3 protein and gene was up-regulated,and the expression of HIF1α protein and gene was down-regulated(P<0.05).Conclusion Yulinzhu can delay ovarian aging by regulating the protein and gene expressions of Sirt3 and HIF1α to improve the antioxidant capacity of ovarian tissue.
NETL
NSTL
万方数据
维普
