Correlation analysis of the effect of Maxing Shigan Decoction on intestinal flora and metabolites in rats with lung injury
Objective To explore the mechanism of action of intestinal flora in the treatment of pneumonia with Maxing Shigan Decoction.Methods A rat model of pneumonia injury was prepared by LPS aerosol inhalation method,and the intestinal content was tested by high-throughput sequencing and metabolomics.The changes in intestinal flora and metabolic products were analyzed after Maxing Shigan Decoction intervention;the KEGG pathway was used as the core for combined analysis of metabolomics and high-throughput sequencing results.Results After treatment with Maxing Shigan Decoction,the animal lung tissue injury was significantly improved.In the intestinal content,dominant bacteria included Staphy-lococcaceae,Gemellaceae,Lactobacillaceae,etc.,and the content of metabolites such as Erucic acid,Succinic acid semial,Eicosadienoic acid was significantly up-regulated.Among them,Veilonella,Aerococcus and other bacteria have a significant correlation with phosphocarbamate,succinic acid hemialdehyde,vitamin D2,palmitic acid,maleic acid,etc.Conclusion Maxing Shigan Decoction can alleviate lung tissue inflammation and injury.Its mechanism of action is related to regulating palm oil acid,maleic acid,phosphoric acid,1-methylhistidine,3-dehydrogenated shikimate acid and other metabolites through the regulation of gut microbiota.
Maxing Shigan Decoctionlung injurylung and stomach responseintestinal florametabolomics
万方数据
维普
