Study on pharmacokinetics of Eucommia ulmoides preparation in rat plasma by UPLC-MS/MS
Objective To etablish a method of ultra high performance liquid chromatography tandem mass spectrometry for the determination of the components of Eucommia ulmoides in plasma,and to study the pharmacokinetics of the components of Eucommia ulmoides and its preparation.Methods After SD rats took orally different Eucommia ulmoides preparations,plasma samples were collected at different time points,and Agilent sb C18 chromatographic column(2.1)was used(2.1 mm× 50 mm,1.8 μm).The mobile phase was consisted of 0.1％formic acid water(A)and 0.1％formic acid acetonitrile(B),and gradient elution was selected;in the multi reaction monitoring(MRM)mode,the dosage forms were measured simultaneously in the negative ion modes;the pharmacokinetic parameters were calculated by Phoenix winnonlin 8.1 software.Results Protocatechuic acid,geniposide,pinoresinol diglucoside,acanthopanax senticosus glycoside,pinoresinol glucoside,geniposide,chlorogenic acid and caffeic acid were in the range of 3.2-2000,3.3-2044,0.6-2028,0.6-1770,0.3-2036,0.6-2004,0.5-1596 and 0.7-1090 ng/mL respectively;specificity,accuracy,precision,stability,recovery and matrix effect all meet the requirements of biological sample verification.Using the non atrioventricular model analysis,the eight components were different in AUC0-∞、t1/2z、Tmax、Cmax、Vz/F、Clz/F、MRT0-∞.Conclusion The method was high sensitivity,specificity and accuracy.It can be used for the pharmacokinetics of Eucommia ulmoides in rats.Different Eucommia ulmoides preparations have different drug release speed,onset speed and action intensity in rats,which has guiding significance in clinical application.
Eucommia ulmoidesUPLC-MS/MSpharmacokinetictraditional Chinese medicinedosage form
万方数据
维普
