Objective To observe the mechanism of fructus mume pills on ulcerative colitis rats,and its compatibility relationship.Methods 83 rats were divided into 8 groups,10 rats in control group,complete receipt group,sour flavour group,bitter flavour group,pungent flavour group,sweet flavour group and SASP group,and 13 rats in model group.UC rats were induced via 4％(v/v)acetic acid enema.The drug was administered 24 hours after molding.Complete rats were orally administrated 900 mg/kg corresponding drug,sour flavour drug group rats were administrated 270 mg/kg,bitter flavour group rats were received 235 mg/kg,pungent flavour group rats were 340mg/kg,sweet group rats were 110mg/kg,and SASP group rats were 300mg/kg.The drug was given intragastric administration at the volume of 10 ml/kg rat body weight,twice a day for seven consecutive days.Control group and model group received equal amount of saline.Disease injury index was used to evaluate UC model,H&E staining were helped to observe pathological damage.Levels of serum inflammatory cytokines IL-6,IL-8,TNF-α and colonic neu-ropeptide levels of 5-HT,ghrelin,vasoactive intestinal peptide,substance P,neuropeptide Y,calcitonin gene-related peptide were detected by ELISA kits.Results(1)Compared with control group,DAI scores in model group increased(P＜0.05).Model group rats colon appeared obvious injuries,including edema,congestion,inflammatory infiltration,and sticking to the surrounding tissue.Serum levels of IL-6,IL-8,TNF-α,were increased(P＜0.05),colonic levels VIP,SP,ghrelin,5-HT,NPY increased and CGRY decreased(P＜0.05).(2)Compared with model group,DAI scores and colon pathology injuries were decreased in complete receipt group,decomposed group,and SASP group.Serum levels of IL-6,IL-8,TNF-αwere reduced in complete receipt,modification groups and SASP group.VIP,SP,ghrelin,5-HT,NPY in colon tissue were decreased and CGPY were increased in complete receipt group,bitter flavour group,and SASP group(P＜0.05).VIP,SP,ghrelin,5-HT,NPY were decreased in sour flavour group(P＜0.05).VIP,ghrelin,5-HT were decreased and SP,CGRY were increased in pungent flavour group(P＜0.05).VIP,SP,ghrelin,NPY were reduced and CGRY was raised in sweet group(P＜0.05).Conclusion Fructus mume pills and its modifications could treat UC via regulating neuropeptides and in-flammation cytokines in Neuro-immune network.The effect of the complete receipt group is obvious,and its modification groups take partial regulation effect on these neuropeptides.All decomposed recipes groups can play anti-inflammatory effects through synergistic effects.
Fructus Mume pillsulcerative colitisneuropeptideinflammation cytokinesdecomposed recipesfive flavors theory
维普
万方数据
