Study on the effect of Yiqi Huayu Jiedu Formula on lung injury and neutrophil adhesion molecules in rats with acute respiratory distress syndrome
Objective To observe the protective effects of the formula of Yiqi Huayu Jiedu Formula and the disassembling formula on lipopolysaccharide(LPS)-induced acute respiratory distress syndrome(ARDS)model rats and the effects of neutrophil adhesion molecules.Methods Thirty-six SD rats were randomly divided into a control group,a model group,a yiqi qingre group,a yiqi huayu group,a yiqi zhuyin group and a whole prescription group of traditional Chinese medicine,with six rats in each group.In the yiqi qingre group,the yiqi huayu group,the yiqi zhuyin group,and the whole formula group,12.2 g/(kg·d)of the corresponding Chinese herbal medicine extract was given by gavage for 7 days,and the rest of the groups were gavaged with an equal amount of distilled water.LPS was injected into the tail vein to establish the ARDS rat model,and the animals in each group were anaesthetised and executed 16 hours after modelling for sampling.Hematoxylin-eosin staining was used to observe the pathological changes in lung tissue,and enzyme-linked immunosorbent assay was used to detect the levels of Rap1b,lymphocyte function-associated antigen-1(LFA-1),macrophage surface antigen-1(LFA-1,macrophage surface antigen-1(MAC-1),and Rap1b in lung tissue by western-blot.Results Compared with the control group,the protein expression of Rap1b,LFA-1 and MAC-1 was up-regulated in the model group.Compared with the model group,Yiqi Huayu Jiedu Formula and the dismantling formula could significantly reduce the lung injury of ARDS model rats,and significantly down-regulate the expression of Rap1b,MAC-1 in alveolar lavage fluid and LFA-1 in lung homogenate.Conclusion The protective effect of Yiqi Huayu Jiedu Formula on LPS-induced ARDS model rats may be related to the reduction of Rap1b,LFA-1 and MAC-1 protein expression levels,and the reduction of neutrophil adhesion activity.
万方数据
维普
