Effect of Qingre Huashi Tiaoshu Recipe on the expression of MUC-2 and Claudin-2 in intestinal mucosa of DSS colitis model mice
Objective Based on the changes of mucoprotein-2(MUC-2)and Claudin-2 expression,this study explored the possible mechanism of Qingre Huashi Tiaoshu Recipe in preventing and alleviating intestinal mucosal barrier damage in dextran sodium sulfate(DSS)colitis model mice.Methods 24 C57BL/6 mice were randomly selected as the normal group,and the remaining mice were given 2.5％DSS aqueous solution to drink freely for 7 days to make molds,and then randomly divided into model group,mesalazine group and Qingre Huashi Tiaoshu Recipe group,with 6 mice in each group.Mice in the normal group and the model group were given 0.5 mL of pure water for gastric gavage in each group;mice in the mesalazine group were given 0.52 g/(kg·d)mesalazine suspension for gavage;mice in the Qingre Huashi Tiaoshu Recipe group were given 1.69 g/(kg·d)gavage.Gastric gavage was performed once a day for 7 days in each group at the same time as molding.After 7 days,the body weight changes of each group were measured and recorded,the disease activity index(DAI)of each group was scored,the colon length of each group was measured,pathological changes of colon tissue were observed,and the ex-pressions of MUC-2 and Claudin-2 in colon tissue were detected.Results Compared with the normal group,body weight,DAI score,colon length and Claudin-2 and MUC-2 protein expressions in colon tissue were significantly decreased in the model group(P＜0.05).Compared with the model group,body weight,DAI score,colon length and MUC-2 protein expression were significantly increased in mesalazine group and the Qingre Huashi Tiaoshu Recipe group(P＜0.05).In terms of the pathological changes of colon tissue,the intestinal mucosal epithelium of mice in the model group was defective,the goblet cells were significantly decreased,the structure of lamina propria and crypt was seriously damaged,the arrangement was disordered,and a large number of lymphocytes infiltrated.There was almost no defect in colonic mucosal epithelium of mice in the heat-clearing and moist-clearing the Qingre Huashi Tiaoshu Recipe group and the mesalazine group,and a few inflammatory cells infiltrated.Conclusion Qingre Huashi Tiaoshu Recipe has certain preventive and palliative effects on intestinal mucosal barrier damage in DSS colitis mice,which may be caused by increasing the expression of MUC-2 in colon tissue,stimulating Claudin-2 secretion and promoting mucosal repair,so as to maintain the homeostasis of intestinal mucosal barrier.
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