Effects of electroacupuncture pretreatment at ST40 on the levels of NGF and NO in rats with cerebral thrombus
Objective To observe the effects of electroacupuncture(EA)pretreatment at Fenglong(ST40)on the behaviors,cerebral infarction volume,levels of infarcted side hippocampal nerve growth factor(NGF)and cortical nitric oxide(NO)of the distal middle cerebral artery thrombus(dMCAT)model induced by FeCl3.Methods 52 adult male SD rats were randomly divided into the sham operation group,the model group,the EA group,and the aspirin group,with 10 rats in the sham operation group and 14 rats in every other group.The rats in the EA group were treated with EA(2/100 Hz,1～3 mA)at bilateral ST40 for 30 minutes once a day for 7 days,while the rats in the aspirin group received aspirin 50 mg/(kg·d)orally for 7 days.On the 8th day,the dMCAT model was induced by 50％FeCl3 to the left middle cerebral artery in model,EA,and aspirin groups.The motor function(Longa scale,screen test,and balance beam test)was evaluated at the 24 hours and the 7 days after operation,and the cerebral infarction volumes was measured by 2,3,5-triphenyl tetrazolium chloride(TTC)staining on the time that 24 hours after the operation.The infarcted side brain tissues of the rats was taken at the 24 hours and the 7 days after operation,and the level of NGF in the hippocampus was measured by the method of Western Blot,while the level of NO in the infarcted cortex was measured by nitrate reductase method.Results Compared with the sham operation group,the levels of Longa score,screen test score,balance beam test score,and the cerebral infarction volume percentage of the model group rats were significantly in-creased 24 hours after the operation(P＜0.01);compared with the model group,24 hours after the operation,the Longa score was decreased in the EA group(P＜0.05),while the screen test score,the cerebral infarction volume percentage were decreased in EA and aspirin groups(P＜0.05,P＜0.01),and the balanced beam test score was decreased in the both groups(P＜0.05),and the expression of NGF in the tissues of hippocampus was increased in EA and aspirin groups 7 days after the operation(P＜0.01,P＜0.05).There was no statistically difference about the cortex NO levels among groups(P＞0.05).Conclusion EA pretreatment could alleviate early brain injury in rats with cerebral thrombosis,improve their motor functions,and increase the level of NGF in the tissues of infarcted hippocampus 7 days after operation,which may be beneficial for later neurological recovery.
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