Study on the mechanism of action of Tuina to improveliver injury in exercise-induced fatigue rats based on RNA sequencing technology
Objective Application of RNA sequencing technology to explore the mechanism of action of Tuina to improve liver injury in exercise-induced fatigue rats.Methods Six-week-old male Sprague-Dawley rats were randomly assigned into the control group,the model group,and the Tuina group,with 8 rats in each group.The control group was fed routinely without any treatment;rats in the model group was rested in a cage after daily exhaustive exercise;and ats in the Tuina group was given Tuina for 15 min after daily exhaustive exercise for a total of 21 days.After sampling,the histopathological methods of light microscopy were used to observe the morphological changes of liver tissues of the three groups of rats,and TUNEL assay was used to observe the apoptosis of rat liver cells;RNA of liver tissues of the three groups of rats was extracted for transcriptome sequencing,and differentially expressed genes were screened and subjected to GO enrichment analysis and KEGG pathway analysis to determine their biological functions.Results Compared with the control group,both groups of rats showed pathological changes of mild liver injury,and the rate of apoptosis in the liver cells of rats in the model group was significantly increased compared with that of the control group(P＜0.05).Compared with the model group,the compared with the model group,the pathological changes in the liver tissue of the Tuina group rats were significantly improved,with slight narrowing of the liver sinusoids and a small amount of inflammatory cell infiltration between lobules.The liver cells tended to be normal,and the rate of apoptosis in the liver of rats in the Tuina group was significantly decreased compared with that in the model group(P＜0.01).After transcriptomic analysis,491 differential genes were screened in the control group and the model group,including 244 up-regulated genes and 247 down-regulated genes.513 differential genes were screened in the model group and the Tuina group,including 257 up-regulated genes and 256 down-regulated genes.There were 5 differential significant genes in both groups,which were the genes of Ppargcla,Fbp2,Gadd45g,Atplb2,and Myh7,respectively.The results of GO enrichment analysis showed that the liver injury of exercise-induced fatigue rats improved by Tuina was mainly involved in signaling,response to stimuli,and nucleic acid binding transcription factor activity,etc.The results of KEGG pathway analysis mainly included FoxO signaling pathway,AMPK signaling pathway,and cGMP-PKG signaling pathway.Conclusion Tuina improved exercise fatigue-induced inflammatory injury and apoptosis in rat liver tissue,and the mechanism may be highly related to Ppargcla,Fbp2,Gadd45g,Atpl b2 and Myh7 genes.
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