The therapeutic effect of Qingwen Baidu Decoction sepsis-induced lung injury in rats and its influence on ferroptosis
Objective To investigate the therapeutic effect and mechanism of Qingwen Baidu Decoction on sepsis-induced lung injury in rats with cecal ligation and puncture(CLP).Methods A total of 75 healthy male SD rats were randomly divided into sham operation group,model group,and low,medium,and high dose Qingwen Baidu Decoction groups,with 30 rats in each group.The sham operation group only underwent laparotomy and suture without CLP and was orally administered with an equal volume of normal saline.The remaining groups underwent CLP to establish a sepsis-induced lung injury rat model.The therapeutic effect of Qingwen Baidu Decoction on sepsis-induced lung injury was investigated by detecting the survival rate,wet-to-dry weight ratio of lung tissue,total protein content and total cell count in bronchoalveolar lavage fluid(BALF),lung tissue lipid peroxidation level,and HE staining after treatment.In addition,the method of western blot was used to detect the expression of FTH,FTL,transferrin,and GPX4 proteins in lung tissue of each group of rats,to explore the effect of Qingwen Baidu Decoction on iron death-related factors in sepsis-induced lung injury rat model,and to preliminarily study its mechanism of action.Results Qingwen Baidu Decoction intervention could effectively reduce the lung W/D ratio,total protein content,and total cell count in BALF of sepsis-induced lung injury rats.It could also decrease the levels of IL-6,IL-1 β,TNF-α,MDA,and 4-HNE in sepsis-induced lung injury rats,reduce the levels of GSSG,and Fe2+,increase the level of T-GSH,GSH,and improve the GSH/GSSG ratio.Moreover,Qingwen Baidu Decoction could reduce lung tissue pathological changes such as alveolar rupture or fusion and inflammatory cell infiltration caused by sepsis-induced lung injury.In addition,Qingwen Baidu Decoction could reduce the protein expression levels of iron death-related factors FTH,FTL and transferrin,while increase the protein expression level of GPX4.Conclusion Qingwen Baidu Decoction has a clear therapeutic effect on sepsis-induced lung injury in rats,and its therapeutic mechanism may be related to the inhibition of iron death.
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