The effect of Linggui Qihua No.2 on the cardiac structure and diastolic function in rats with HFpEF
Objective To observe the effect of Linggui Qihua No.2(LGQH2)on the cardiac structure and diastolic function in rats with heart failure with preserved ejection fraction(HFpEF)rats.Methods Ten 7-week-old WKY rats were used as the normal group,and 40 SHR rats were used to establish HFpEF model with composite diabetes,hypertension,and hyperlipidemia.Three rats were died at this stage.After successful modeling,all rats were randomly divided into the model group,the empagliflozin group,the low-and the high-dose decoction groups.The WKY and the model group received an equal dose of normal saline.The intervention groups were administered empagliflozin[1.8 mg/(kg·d)],low-dose[8.1 g/(kg·d)]and high-dose decoction[16.2 g/(kg·d)],respectively,for 4 weeks.At the end of the intervention,echocardiography was used to measure left ventricular end-diastolic internal diameter(LVEDD),left atrial internal diameter(LAD),right atrial internal diameter(RAD),interventricular septal thickness(IVST),and the ratios of left ventricular ejection fraction(LVEF),isovolumic diastolic time(IVRT),and the ratio of the early diastolic mitral blood flow rate(E)to mitral annular myocardial motion velocity(e').The level of atrial and brain natriuretic peptide in serum were measured using enzyme-linked immunosorbent assay.Pathological changes in left atrial myocardial tissue were observed by HE staining,and myocardial tissue fibrosis was observed with Masson staining.Results Compared with the WKY group,The rats in the model group showed shortness of breath,decreased appetite and irritability.The LVEDD,LAD,RAD,IVST,E/e',IVRT,as well as the level of ANP and BNP were significantly increased in the model group(P<0.01).Compared with the model group,the rats in each treatment group were in good spirits,responsive,and took more food and water.The LVEDD,LAD,RAD,IVST,E/e'and IVRT were improved significantly in the administration groups and the level of ANP and BNP were significantly decreased(P<0.05).Conclusion LGQH2 decoction may improve cardiac remodeling and diastolic dysfunction in HFpEF rats by attenuating myocardial hypertrophy and fibrosis,and become a valuable prescription for HFpEF.
Linggui Qihua No.2heart failure with preserved ejection fractionstructure and function of the heartdiastolic dysfunctionatrial natriuretic peptideB-type brain natriuretic peptidemorphology of pathology
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