Ultrasonic layer-specific strain imaging for evaluating myocardial function in rats with early stage of heart failure with preserved ejection fraction by Linggui Qihua Formula
Objective To assess the efficacy of the Linggui Qihua Formula in enhancing myocardial function in early-stage rats with heart failure with preserved ejection fraction(HFpEF)using ul-trasonic layer-specific strain imaging technology.Methods 40 SPF-grade SHR rats were randomly assigned to the HFpEF model group,empagliflozin group,low-dose Linggui Qihua Formula group,and high-dose Linggui Qihua Formula group,while 10 WKY rats were assigned to the control group.The early-stage rat model of HFpEF was established by a high-salt,high-fat,and high-sugar diet along with intraperitoneal injection of streptozotocin.After 9 weeks of respective interventions,measurements were taken for left ventricular end-diastolic diameter(LVEDd),left ventricular posterior wall thickness(LVPWd),E/e',left ventricular ejection fraction(LVEF),as well as global longitudinal strain(GLS)and global circumferential strain(GCS)at the endocardium(endo),midmyocardium(mid),and epicardium(epi)of the left ventricle.The levels of BNP in serum were detected by the method of enzyme-linked immunosorbent assay.The changes in myocardial tissue were observated using masson staining.Results Compared with the model group,the levels of GLSendo,GLSmid,and GCSendo showed a significant increase in the empagliflozin group(P<0.05).The levels of GLSendo and GCSmid were significantly improved in the low-dose group of Linggui Qihua Formula(P<0.05).The levels of GLSendo,GLSmid and GCSmid exhibited a significant increase in the high-dose group of Linggui Qihua Formula(P<0.05).The intercellular gap of cardiomyocytes were decreased while collagen fiber deposition were reduced in both the low-dose and the high-dose groups.Conclusion The Linggui Qihua Formula can enhance the levels of GLSendo,GLSmid,and GCSmid in the early-stage HFpEF model rats,improving left ventricular remodeling and systolic function.The application of ultrasonic layer-specific strain imaging technology enables non-invasive,quantitative,and sensitive detection of myocardial changes in the left ven-tricular wall of early-stage HFpEF rats.
early stage of heart failure with preserved ejection fractiontwo dimensional speckle tracking echocardiographylayer-specific strainLinggui Qihua Formulamyocardial function
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