Establishment of specific chromatogramfor sesquiterpene lactones and quantitative study on multi components in Centipeda minima
Objective To evaluate the difference of Centipeda minima from different producing areas by establishing HPLC specific chromatogram and determining the contents of four effective components of Centipeda minima. Methods The HPLC Method was adopted. The determination was performed on a column of Waters HSS T3 (4.6 mm×250 mm,5 μm) with acetonitrile-0.02％ phosphoric acid solution as the mobile phase by gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was 225 nm,the column temperature was 40 ℃,the injection volume was 10 μL. The HPLC specific chromatograms of 20 batches of Centipeda minima were established and the common peaks were identified by reference substances and UV 3D spectral analysis. The contents of four components were determined. Similarity evaluation and principal component analysis ( PCA ) were carried out on the fingerprints and orthogonal partial least squares discriminant analysis ( OPLS-DA ) was used to find the different components of Centipeda minima from different producing areas. Results There were 7 common peaks in the specific chromatograms of Centipeda minima by comparing with the reference substances,three common peaks were identified,namely,caffeic acid,ferulic acid and isoferulic acid. Except for three batches of samples from Guangxi Province,the similarity of the other 17 batchs were greater than 0. 95. According to the analysis of PCA,20 batches of Centipeda minima were divided into two categories. Three kinds of biomarkers were determined by OPLS-DA method. The order of significance was peak 6>Arnicolide C>peak 7,respectively. Thecontents of Arnicolide C in samples from the Guangxi were significently higher than that in samples from other areas. Conclusion This method can effectively analyze the quality differences of Centipeda minima from different producing areas and provide reference for the quality evaluation.
Centipeda minimaHPLC specific chromatogrammulti-component determinationdifference markers
万方数据
维普
