Study on the toxicity in normal rats of Euphorbiae Pekinensis Radix before and after processing with vinegar
Objective To compare the differences of hepatic,gastrointestinal and renal toxicities of Euphorbiae Pekinensis Radix on normal rats before and after processing with vinegar. Methods 56 male SD rats were randomly divided into 7 groups:blank group,low,middle and high dose groups of Euphorbiae Pekinensis Radix and vinegar-processed Euphorbiae Pekinensis Radix. The rats were orally ad-ministered with powder of Euphorbiae Pekinensis Radix and vinegar-processed Euphorbiae Pekinensis Radix at the dose of 0.253,0.506,1.012g/kg for seven consecutive days. The blank group was treated with an equal volume of 0.5％ carboxy methyl cellulos sodium. Pathological sections were prepared to observe the tissue damages in rats. The liver and kidney function indexes in serum and the oxidative damage indexes in serum and tissues were investigated. Results Compared with the blank group,different degrees of tissue damage were seen in the pathological sections of liver,stomach and intestine of rats in each administration group. For the group of Euphorbiae Pekinensis Radix,the activity of alanine aminotransferase ( ALT) and aspartate transaminase (AST) in serum increased significantly(P﹤0.01,P﹤0.05),the creatinine (CRE) and urea nitrogen ( BUN) increased slightly. The contents of superoxide dismutase ( SOD) and glutathione (GSH) in serum and tissues decreased remarkably,but malondialdehyde (MDA) increased(P﹤0.01,P﹤0.05). Compared with the Euphorbiae Pekinensis Radixgroups,slight injuries on liver,stomach and intestine of rats treated with vinegar-processed Euphorbiae Pekinensis Radix were seen,and the activities of ALT and AST were reduced significantly. Meanwhile,the levels of SOD and GSH in serum and tissues significantly increased,while MDA signifcantly decreased(P>0.05),but there were no significant differences between the two groups. Conclusion The toxicity of Euphorbiae Pekinensis Radix on the liver,stomach and intestine of normal rats was obvious,no obvious damage on the kidney. The toxicity decreased after processing with vinegar,which may be related to the oxidative damage.
Euphorbiae Pekinensis Radixprocessing with vinegarnormal rattoxicitypathological changeliver and kidney functionoxidative damage indexes
万方数据
维普
