Multimodal magnetic resonance imaging study on Buyang Huanwu Decoction improving cerebral blood flow in cerebral ischemic rats
Objective This study aimed to observe the effects of Buyang Huanwu Decoction(BHD)on collateral circulation and cerebral blood flow in rats with cerebral ischemia using multimodal magnetic resonance imaging(MRI)techniques.Methods The permanent middle cerebral artery occlusion(MCAO)model was established using the suture method in rats.A total of 24 rats were randomly divided into the sham group,model group,BHD high-dose group(16.6 g/kg),and BHD low-dose group(8.3 g/kg),8 rats per group.Rats in the treatment groups received oral administration of BHD or an equal volume of saline for 15 consecutive days,starting 24 hours after the surgery.Multimodal MRI was performed on the 7th and 15th day following ischemia onset.T2-weighted imaging was used to measure the infarct volume.Magnetic resonance angiography was performed to evaluate the signal intensities of the anterior cerebral artery(ACA),internal carotid artery(ICA),posterior cerebral artery(PCA),and basilar artery(BA).Arterial spin labeling was applied to assess cerebral blood flow at the periinfarct area.Results Compared with the sham group,the model group showed a significant decrease in signal intensity of the ACA,ICA,and PCA(P<0.05,P<0.001)and a significant increase in signal intensity of the BA(P<0.001)on days 7 and 15 after ischemia.Additionally,there was a significant reduction in cerebral blood flow at the periinfarct area(P<0.001).Compared with the model group,both high-dose(16.6 g/kg)and low-dose(8.3 g/kg)BHD treatments significantly reduced the infarct volume on day7 after ischemia(P<0.05),decreased the signal intensity of the BA on days7 and15(P<0.05,P<0.01),and increased the cerebral blood flow at the periinfarct area on day 15(P<0.001).Furthermore,the high-dose BHD treatment also reduced the infarct volume(P<0.01)and increased the signal intensity of the ACA and ICA(P<0.05)on day 15 after ischemia,while the low-dose BHD treatment increased the cerebral blood flow at the ischemic border on day 7 following ischemia(P<0.05).Conclusion BHD could promote the establishment of collateral circulation in the key intracranial arteries,improve cerebral blood flow,and reduce infarct volume,thereby protecting the brain.
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