Effects of total saponin of Trillium tschonoskii Maxim.on cortex microstructure and amino acid neurotransmitters in rats with cerebral ischemia
Objective To observe the effects of total saponin of Trillium tschonoskii Maxim(TSTT)on cortex microstructure and amino acid neurotransmitters in rats with cerebral ischemia;To explore its pharmacological of against cerebral ischemia.Methods SD rats were randomly divided into sham-operation group,model group and TSTT 33,65,130 mg/kg dosage group,with 8 rats in each group.Rat model of middle cerebral artery occlusion was established with the suture method.Rats in the above groups received respectively intervention for 15 days.The transmission electron microscope was used to detect the ultrastructure of neurons,mitochondria,and synapses in cortex.OPA-precolumn derivatization high performance liquid chromatography with fluorescence detection was used for the determination of glutamic acid(Glu)and γ-aminobutyric acid(GABA)in the cortex of each rat.Results The results of transmission electron microscopy showed that the microstructure of neurons and mitochondria was damaged,the number of synapses was decreased,the synaptic gap was blurred,and the length of postsynaptic density(PSD)was shortened and the thickness was thinned.Compared with the model group,the neuronal score in the TSTT 65 mg/kg group(P<0.01)was significantly decreased,and the neuronal nuclear membrane was more complete.The mitochondrial score of the TSTT 130 mg/kg,65 mg/kg and 33 mg/kg groups were significantly decreased(P<0.01).The degree of mitochondrial bilayer membrane damage was decreased,and the internal ridge structure was still visible.TSTT 130 mg/kg significantly increased the number of synapses(P<0.01).TSTT 65 mg/kg and 33 mg/kg significantly increased the number of synapses,PSD length and thickness(P<0.01).The results of high performance liquid chromatography showed that the ratio of Glu/GABA in the cortex of the model group was significantly higher than that in the sham operation group(P<0.05).The content of GABA in the cortex of TSTT 65 mg/kg rats was significantly increased,and the ratio of Glu/GABA was significantly decreased(P<0.05).Conclusion TSTT can protect the brain by reducing the structural damage of mitochondria,neurons and synapses in rats with cerebral ischemia,down-regulating the ratio of Glu to GABA neurotransmitter amino acids and improving synaptic plasticity.
total saponin of Trillium tschonoskii Maxim.cerebral ischemiasynaptic plasticityGlu/GABA
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