Exploration of the effect of Yiqi Kangfei Prescription on oxidative damage in the lungs of golden hamsters with lipopolysaccharide-induced acute lung injury based on the Nrf2/HO-1 signaling path-way
Objective Based on the Nrf2/HO-1 signaling pathway,this study explored the effects and mechanisms of Yiqi Kangfei Prescription on oxidative damage in the lungs of golden hamsters with li-popolysaccharide(LPS)-induced acute lung injury(ALI).Methods Sixty SPF Syrian golden hamsters were randomly divided into blank group,model group,dexamethasone group,low-dose Yiqi Kangfei Prescription group,medium-dose Yiqi Kangfei Prescription group,and high-dose Yiqi Kangfei Prescription group,with 5 females and 5 males in each group.Before modeling,preventive medication was given.Rats in the blank group and the model group were given an equal volume of saline by gavage.Rats in the dexam-ethasone group were given an equal amount of dexamethasone sodium phosphate injection by continuous in-traperitoneal injection for 4 days before modeling.Rats in the low,medium,and high-dose Yiqi Kangfei Prescription groups were given the corresponding doses of traditional Chinese medicine suspension by continuous gavage for 14 days before modeling.Two days after the end of preventive medication,ALI hamster models were established by intratracheal instillation of LPS,and tissues were dissected 48 hours after modeling.The level of the lung lavage fluid(BALF)was detected by the BCA method,and the lung index of hamsters was calculated.The method of HE staining was used to detect pathological changes in lung tissue;fluorescence probes were used to detect ROS levels,the TBA method was used to detect MDA levels,the WST-1 method was used to detect SOD activity in lung tissue,the colorimetric method was used to detect GSH-PX activity,and the method western blotting was used to detect Nrf2 and HO-1 protein expression levels in lung tissue.Results Compared with the blank group,the lung index and protein con-centration in BALF of the model group were increased,lung tissue pathological damage was severe,ROS and MDA levels were elevated,SOD and GSH-PX activities were decreased,and Nrf2 and HO-1 protein content were decreased.After drug intervention,compared with the model group,the lung index and protein concentration in BALF of the dexamethasone and traditional Chinese medicine groups were decreased,lung tissue pathological damage was lighter,ROS and MDA levels were decreased,SOD and GSH-PX activities were increased,and Nrf2 and HO-1 protein content were increased,especially in the traditional Chinese medicine high-dose group,the therapeutic effect was most significant(P<0.05).Conclusion Yiqi Kangfei Prescription may regulate the Nrf2/HO-1 pathway,enhance SOD and GSH-PX activity,and inhibit inflammation and oxidative damage caused by LPS.
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