Effects of sofren injection on hypoxic H9C2 cardiomyocytes based on PI3K/AKT/mTOR pathway
Objective To investigate the effect and protective mechanism of sofren injection in hypoxic H9C2 cardiomyocytes.Methods H9C2 cardiomyocytes were simulated by hypoxia.The experiment was divided into normal group,model group,sofren injection group(SI group)and nicodil group.mRNA levels of phosphoinositide 3-kinase(PI3K),protein kinase B(AKT)and mammalian target of rapamycin(mTOR)were detected by RT-qPCR.The protein expression of PI3K,AKT,mTOR,p-PI3K,p-AKT and p-mTOR were detected by Western Blot.The level of apoptosis was detected by flow cytometry.Results Compared with the model group,the mRNA expressions of PI3K,AKT and mTOR in the SI group and the nicodil group were significantly decreased(P<0.05).Compared with the model group,there were no significant differences in PI3K,AKT and mTOR protein expressions between the SI group and the nicodil group(P>0.05).After phosphorylation,the protein expressions of p-PI3K,p-AKT and p-mTOR in SI the group and the nicodil group were significantly decreased compared with the model group(P<0.05).Compared with the nicodil group,the protein expressions of p-PI3K and p-AKT in the SI group were decreased(P<0.05),while the protein content of p-mTOR was not significantly different(P>0.05).Conclusion Sofren injection can suppress excessive autophagy of hypoxic cardiomyocytes and ameliorate cardiac cell damage via downregulating the PI3K/AKT/mTOR pathway.
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