质谱分析联合网络药理学和动物实验探讨健脑固本方治疗脑小血管病性认知障碍的作用机制
Mass spectrometry analysis combined with network pharmacology and animal experiments to investigate the mechanism of action of Jiannao Guben Formula in the treatment of cerebral small vessel disease cognitive impairment
曾伟坤 1韩振蕴 2胡玉立 2王育纯 3田丹枫 4常泽5
作者信息
- 1. 100078 北京中医药大学东方医院
- 2. 北京中医药大学深圳医院(龙岗)脑病科
- 3. 中国医药科学出版社
- 4. 100078 北京中医药大学东方医院体检科
- 5. 中国中医科学院西苑医院脑病科
- 折叠
摘要
目的 运用质谱分析、网络药理学和动物实验探讨健脑固本方治疗脑小血管病性认知障碍(cerebral small vessel disease,CSVCI)的作用机制.方法 通过质谱分析得出主要活性成分,TCMSP检索其相应作用靶点;通过GeneCards、OMIM数据库检索CSVCI疾病靶点;通过String数据库和Cytoscape软件构建药物疾病作用靶点网络可视化;利用DAVID数据库对交集靶点进行GO功能和KEGG信号通路富集分析.结果 健脑固本方的有效活性成分有山奈酚、薯蓣皂苷,作用于丝裂原活化蛋白激酶8、蛋白激酶B1 等核心靶点,可能通过磷脂酰肌醇3-激酶/蛋白激酶B等信号通路涉及炎症反应.蛋白免疫印迹法检测结果表明健脑固本方抑制Notch-1、RBP-Jκ以及Hes-1 的表达.结论 本研究初步表明健脑固本方能够通过多靶点、多通路、多途径治疗CSVCI,具体作用机制可能是抑制Notch-1 信号通路,进而抑制炎症反应.
Abstract
Objective To investigate the mechanism of action of Jiannao Guben Formula for the treatment of cerebral small vessel disease cognitive impairment(CSVCI)with the method of mass spectrometry,network pharmacology and animal experiments.Methods The main active ingredients were identified by mass spectrometry,and their corresponding targets were searched by TCMSP database;the targets of CSVCI were searched by GeneCards and OMIM databases;the network of drug targets was visualised by String database and Cytoscape software;the intersecting targets were analyzed by GO and KEGG using DAVID database.Results The active ingredients,including kaempferol and diosgenin,acted on the core targets of MAPK8 and AKT1,and may be involved in the inflammatory response through the PI3K-Akt signalling pathway,etc.The results of WB showed that Jiannao Guben Formula inhibited the expression of Notch-1,RBP-Jκ and Hes-1.Conclusion Jiannao Guben Formula can pass through multiple targets and pathways to treat CSVCI,and it may inhibit the inflammatory response through the Notch-1 signaling pathway for the treatment of CSVCI.
关键词
质谱分析/网络药理学/健脑固本方/脑小血管病性认知障碍/Notch-1信号通路/炎症反应Key words
mass spectrometry analysis/cyberpharmacology/Jiannao Guben Formula/CSVCI/Notch-1 signalling pathway/inflammatory response引用本文复制引用
基金项目
国家自然科学基金面上项目(82274466)
北京中医药大学东方医院2023年度基本科研业务费(新教师启动基金)(2023-JYB-XJSJ047)
出版年
2024
国家科技期刊平台
NSTL
万方数据