Study on the mechanism of Soufeng Yuchuan Decoction and split decoction on airway inflammation and airway hyperresponsiveness in asthmatic rats
Objective To investigate the mechanism of Soufeng Yuchuan Decoction and dismantling decoction on airway inflammation and airway hyperresponsiveness in asthmatic rats.Methods Twenty-eight rats were randomly divided into the normal group,model group,dexamethasone group,Soufeng Yuchuan Decoction group,Qufufeng Decoction group,Xiaoanyu Decoction group and Qusutan Decoction group;in addition to the normal group,the rat model of asthma was established by sensitization with injection and atomization of ov-albumin(OVA),and Soufeng Yuchuan Decoction and dismantling decoction were used for intervention.The serum levels of interleukin-6(IL-6),interleukin-13(IL-13),transforming growth factor β1(TGF-β1)and tumor necrosis factor α(TNF-α)were detected by enzyme-linked immunoassay(ELISA).HE staining and immunohistochemistry were used to observe the pathological morphology of airway and lung tissues.The mRNA expression of IL-6,IL-13,TGF-β1 and TNF-α in rat lung homogenate was detected by polymerase linked reaction(PCR).Airway hyperreactivity of asthmatic rats was detected by pulmonary function in vitro.Results Compared with the normal group,the asthmatic manifestations of rats in the model group were obvious,and a large number of inflammatory cells could be seen around the bronchus and lung tissues,the bronchial tube wall was thickened,the serum IL-6,IL-13,TGF-β1,TNF-α contents and mRNA expression were significantly increased(P＜0.05).Airway hyperreactivity was enhanced(P＜0.05);compared with the model group,the asthma manifestations,inflammatory cell infiltration in airway and lung tissue in the dexamethasone group,Soufeng Yuhuan Decoction group,Qufufeng Decoction group,Xiaoanyu Decoction group and Qusutan Decoction group were decreased.The bronchial tube wall were slightly thickened.The levels of IL-6,IL-13,TGF-β1,TNF-α content and mRNA expression were significantly decreased(P＜0.05).Airway hyperreactivity was significantly decreased(P＜0.05);Compared with other Chinese medicine groups,Soufeng Yucuan Decoction group was superior to the other groups in reducing airway inflammation,improving lung tissue pathological injury,improving airway hyperresponsiveness,and reducing serum IL-6,IL-13,TGF-β1,TNF-α content and mRNA expression(P＜0.05).Conclusion Soufeng Yuchuan Decoction and its dismantling decoction can reduce the expression of IL-6,IL-13,TGF-β1,TNF-α,and improve airway hyperreactivity of asthma.Compared with the Xiaoanyu Decoction group and Qusutan Decoction group,the Qufufeng Decoction group has advantages in improving airway inflammation and reducing airway hyperreac-tivity.
万方数据
维普
