Effect of electroacupuncture at Zusanli and Taichong points on gastrointestinal motility and NO/sGC/cGMP/PKG1 pathway in rats with functional dyspepsia
Objective To investigate the effects of electroacupuncture on gastrointestinal motility and NO/sGC/cGMP/PKG1 pathway in FD rats.Methods The 40 SPF grade male SD rats were divided into the control group,the model group,the EA group,the ODQ group,and the EA+ODQ group,with 8 rats in each group.After modelling by the multifactorial intervention method,the EA group was selected to receive electroacupuncture at the Zusanli and Taichong points for 30 min once a day for 10 days.The ODQ group was injected intraperitoneally with the inhibitor ODQ solution(2 mg/kg)every day for 10 days;the EA+ODQ group was followed after intraperitoneal injection of an equal amount of inhibitor;no special inter-vention was performed in the control group and the model group.Gastric residual rate and small intestinal propulsion rate were detected after the intervention,HE staining was used to observe the morphology of gastric sinus tissue,and Western blot and RT-qPCR were used to detect the expression levels of sGC,cGMP,and PKG1 proteins and mRNA in gastric sinus tissues.Results No obvious organic changes were observed in the gastric sinus tissue of rats in all groups.Compared with the control group,the gastric residual rate was increased,the intestinal propulsion rate was decreased,and the expression of sGC,cGMP,PKG1 protein and mRNA in the gastric sinus tissue was significantly decreased(all P＜0.01).Compared with the model group,the gastric residual rate in the EA,ODQ and EA+ODQ groups was significantly decreased,and the small bowel propulsion rate was significantly increased,and the sGC,cGMP,PKG1 protein and sGC,cGMP mRNA expression were increased(all P＜0.05).The sGC,cGMP,PKG1 protein,and sGC,cGMP mRNA expression in gastric sinus tissue were increased in the EA group and the EA+ODQ group compared with the ODQ group(all P＜0.05).Conclusion Electroacupuncture improves gastrointestinal dyskinesia in FD rats,and the mechanism may be related to the modulation of NO/sGC/cGMP/PKG1 pathway.
万方数据
维普
