Objective Based on the dynamic changes of anti-inflammatory and pro-inflammatory factors and observation under electron microscope to explore the mechanism of action of Fuzheng Touxie Jiedu Huayu Formula on MDRPA.Methods The experimental elderly rats were randomly divided into a blank control group,a model control group,a Western medicine control group,a Chinese medicine group,a Chinese medicine early administration group,and a combination of Chinese and Western medicine treatment group.The dynamic changes of anti-inflammatory and pro-inflammatory factors in each group of elderly rats at different time points were dynamically observed.Compare the ultrastructural changes of lung tissue under electron microscopy in different groups.Results The combination of traditional Chinese and Western medicine can significantly reduce the overexpression of pro-inflammatory cytokine interleukin-6(IL-6)and high mobility group protein 1 in the lung tissue of the target organ of an elderly rat pneumonia model caused by multidrug-resistant Pseudomonas aeruginosa infection,promote the expression of anti-in-flammatory cytokine IL-10,and have a stronger effect than the Western medicine group(P＜0.05,P＜0.01).In the early stage of inflammatory response,the Zhongti group can increase the expression of anti-inflammatory cytokine IL-10 in target organ lung tissue(P＜0.05,P＜0.01),and significantly reduce the expression of pro-inflammatory cytokine IL-6 and high mobility group protein 1(P＜0.05,P＜0.01).Observation under electron microscope:In the model group,exudation appeared in the alveolar cavity,and the alveolar capillaries were dilated and congested;The Western medicine group showed mild fibrosis in the alveolar wall and exudation in the alveolar cavity;The traditional Chinese medicine group showed mild thickening of the alveolar wall and no obvious exudation in the alveolar cavity;The group receiving early administration of traditional Chinese medicine showed mild thickening of the alveolar wall and no significant exudation in the alveolar cavity.Conclusion The Fuzheng Touxie Jiedu Huayu Formula can regulate the inflammatory response of early infected elderly rats,and the combination of traditional Chinese and Western medicine can correct the disordered inflammatory state after infection with MDRPA.Early administration of traditional Chinese medicine canplay a certain preventive and therapeutic role in the inflammatory response after infection with multi drug resistant Pseudomonas aeruginosa.
维普
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