Effect of warming kidney and strengthening spleen and promoting blood circulation and eliminating turbidity decoction on TGF-β1-Smad2/3 signaling pathway in kidney tissue of rats with chronic kidney disease
Objective To investigate the effect of Professor Jin Hongyuan's warming kidney and strengthening spleen and promoting blood circulation and eliminating turbidity decoction on transforming growth factor in kidney tissue of rats with chronic kidney disease(CKD)and to discuss the role of TGF-β1-Smad2/3 signaling pathway.Methods 56 rats were randomly divided into the blank group with 13 rats,and the remaining 43 rats were given adenine solution to prepare CKD rat model.After modeling identification,10 rats in the blank group were selected as the blank control group,and 40 rats with successful modeling were randomly divided into the model group,low,medium and high dose groups of traditional Chinese medicine with 10 rats in each group.The concentrations of the low,medium and high dose groups were administrated by gavage in the dose of 9.18 g/(kg·d),18.36 g/(kg·d)and 36.72 g/(kg·d),respectively.The rats in the blank control group and the model group were given equal volume of normal saline for 4 weeks.After intervention,the general condition of rats was observed.The quantitative levels of urine protein,creatinine,TGF-β1and serum TGF-β1,cystatin C and creatinine in 24 hours were detected by enzyme-linked immunosorbent assay.The pathological changes of kidney were observed by hematoxylin-eosin and Masson staining.The protein expression levels of TGF-β1,Smad2,Smad3,p-Smad2,p-Smad3 and Smad7 in rat renal tissue were detected by Western blot.Results Compared with the model group,the levels of TGF-β1,creatinine and 24-hour urinary protein in urine of rats in the low,medium and high dose groups of traditional Chinese medicine were significantly decreased(P＜0.05).The quantitative levels of TGF-β1,cystatin C and creatinine in serum were sig-nificantly decreased,and the quantitative levels of TGF-β1,cystatin C and creatinine in serum of rats in the high,medium and low dose groups of traditional Chinese medicine were decreased with the increase of dose,with a negative correlation,and the difference was statistically significant(P＜0.05).Compared with the model group,the protein quantity of TGF-β1,Smad2 and Smad3 in renal tissue of rats in the low,medium and high dose groups of traditional Chinese medicine was significantly decreased,and decreased with the increase of Chinese medicine dose,but the difference was not statistically significant.Smad7 increased with the increase of Chinese medicine dose,and the difference was statistically significant(P＜0.05),with a positive correlation.The results of hematoxylin-eosin staining showed that the degree of renal tubule lesion and fibrosis area of rats in each dose group were significantly reduced than those in the model group.Compared with the model group,the renal tissue structure of rats in the low,medium and high dose groups of traditional Chinese medicine were decreased,the inflammatory cell infiltration of renal interstitial decreased and the fibrous tissue decreased.Compared with the low dose group of traditional Chinese medicine,the inflammatory cell infiltration of renal tissue was slightly reduced in the medium and high dose groups of traditional Chinese medicine,and the structure of renal tissue was slightly orderly.The results of masson staining showed that compared with the model group,the proliferation degree of renal interstitial fiber tissue was reduced in the three groups of low,medium and high doses groups of traditional Chinese medicine,and the proportion of fibrosis was reduced,but the difference of renal interstitial fiber tissue proliferation degree was not obvious among the groups.Conclusion Warming the kidney and strengthening the spleen and activating the blood to relieve turbidness can effectively reverse the renal tissue injury in rats with CKD,and it may play a role by down-regulating the level of TGF-β,through the TGF-β1-SMad2/3 pathway,and then improve the renal tissue fibrosis in rats with CKD.
chronic kidney diseasewarming kidney and strengthening spleen and promoting blood circulation and eliminating turbidity decoctionrat kidney tissuekidney functionrenal fibrosisTGF-β1-Smad2/3
万方数据
维普
