Exploring the effect of single abdominal massage on acute alcoholic liver injury in mice
Objective This study aims to explore the effects of a single session of hepatic area massage on mice with acute alcoholic liver injury.Methods Twenty-four 8-week-old male C57BL/6 mice were randomly divided into four groups:the control group,the model group,the 6-hour massage group,and the 24-hour massage group,with six mice in each group.Acute alcoholic liver injury was induced by administering 75％ethanol via gavage at a dose of 12 μL/g twice,with a 12-hour interval between adminis-trations.After the model was established,the mice in the 6-hour and 24-hour massage groups received a single 5-minute hepatic area massage.Tissue samples were collected 6 hours post-intervention from the model,control,and 6-hour massage groups,and 24 hours post-intervention from the 24-hour massage group.Liver-to-body weight ratios were calculated;The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD),glutathione(GSH),and ma-londialdehyde(MDA)in serum and liver homogenates were measured.The liver tissue morphology was observed using hematoxylin-eosin(HE)staining,and fat degeneration in liver cells was examined with Oil Red O staining.Results(1)Compared with the control group,the levels of liver indices,AST,ALT in the model group were increased(P＜0.05).The 6-hour massage group exhibited reduced ALT and AST levels compared with the model group(P＜0.05),and the 24-hour massage group showed reductions in liver index,ALT,and AST(P＜0.05).(2)HE staining:Liver cells in the model group displayed disorganized hepatic cords and damaged lobular structures.Both the 6-hour and 24-hour massage groups showed significant reductions in liver cell damage,with more organized hepatic cords.(3)Compared with the control group,the model group exhibited increased TG and decreased HDL-C(P＜0.05).The 6-hour massage group showed decreased TG and increased HDL-C(P＜0.05),while the 24-hour massage group had reduced TG and LDL-C,and increased HDL-C(P＜0.05).(4)There was an increase in lipid droplets in liver cells of the model group,while the extent of fatty degeneration was significantly reduced in both the 6-hour and 24-hour massage groups.(5)The model group showed decreased GSH and increased SOD levels compared with the control group(P＜0.05).No significant differences were observed in the levels of SOD,MDA,or GSH levels between the massage groups and the model group(P＞0.05).Conclusion Single session hepatic area massage offers protective effects against acute alcoholic liver injury in mice and improves liver function markers and lipid levels,reducing pathological liver damage and fatty degeneration of liver cells.The effects are durable,demonstrating the potential therapeutic benefits of this intervention.
single abdominal massageacute alcoholic liver injurylipid metabolismliver protection
万方数据
维普
