The effects of moxa combustion products on cognitive function and monoamine neurotransmission in the hippocampus and entorhinal cortex of APP/PS1 mice
Objective To investigate the effects of moxa combustion products intervention on the neuronal morphology and neurotransmitter content in the hippocampus(HPC)and entorhinal cortex(EC)of amyloid precursor protein/presenilin 1(APP/PS1)mice,and to explore the mechanisms of moxa combustion products on Alzheimer's disease.Methods 4-month-old male APP/PS1 mice were randomly divided into the model group,the moxa combustion products group,and the essential oil group,and male C57BL/6 mice of the same age were set up as the control group,with 9 mice in each group.Mice in the essential oil group was given 1％rosemary essential oil for sniffing.Mice in the moxa combustion products group received an exposure of 10～15 mg/m3 of moxa combustion products.Mice in control and the model groups were captured and fixed daily without any intervention.All interventions were conducted once a day for 20 minutes,6 days a week for 12 weeks.At the end of the experiment,the learning memory level of mice in each group was detected using the novel object recognition test,the morphology of neurons in the HPC and EC regions of mice in each group was observed using HE staining,and dopamine(DA),5-hydroxytryptamine(5-HT),and norepinephrine(NE)levels in the two regions were measured using ELISA.Results In the novel object recognition experiment,mice in the moxa combustion products group and the essential oil group had increased indices of new object recognition and discrimination compared with the model moxa combustion products group(P＜0.01);the result of HE staining showed that,there was an improvement in neuronal form of HPC and EC in mice in the moxa combustion products group and essential oil group compared to the model group;the result of ELISA showed that,both the DA,5-HT and NE levels in the HPC and EC regions of mice in the essential oil group were elevated compared with the model group(P＜0.05),and the 5-HT,NE and DA levels in both the HPC and EC regions of mice in the moxa combustion products group were elevated(P＜0.05).Conclusion Moxa combustion products intervention alleviated neuronal form damage in both HPC and EC regions of APP/PS1 mice and increased 5-HT,NE and DA levels in both regions,thereby improving their cognitive functions.
万方数据
维普
