Study on the effect of Buyang Huanwu Tang combined with endothelial progenitor cell transplantation on reducing cerebral ischemia-reperfusion injury
Objective To investigate the effect of Buyang Huanwu Tang combined with endothelial progenitor cells(EPCs)on cerebral ischemia-reperfusion.Methods EPCs were first cultured from C57BL/6 mice at 5 weeks of age.Then,30 C57BL/6 mice(6～8 weeks of age)were divided into two groups:a normal control group(6 mice)and a MCOA/R model was constructed and interventions were applied with EPCs transplantation and/or Buyang Huanwu Tang.The successfully modeled mice were randomly divided into MCOA/R group,EPCs group,Buyang Huanwu Tang group,and Buyang Huanwu Tang combined with EPCs group,with 6 mice in each group.24 hours after model establishment,EPCs and/or Buyang Huanwu Tang were injected into the mice via tail vein and administered orally for a total duration of 7 sessions.The infarct area was evaluated by neurological function scoring,staining with 2,3,5-triphenyltetrazolium chloride(TTC),immunohistochemical analysis of Ki67 expression,colorimetric detection of serum superoxide dismutase(SOD)and malondialdehyde(MDA)levels,and Western blotting(WB)detection of NAD(P)H quinone oxidoreductase 1(NQO1),Bcl-2,and Bax expression in mouse brain tissue.Results Compared with the MCOA/R group,each treatment group significantly improved the neurological function score of mice(P＜0.05),reduced cerebral infarction area(P＜0.01),increased Ki67 expression(P＜0.01),increased SOD levels and decreased MDA levels(P＜0.01),increased NQO1 expression(P＜0.01),and increased Bcl-2 and decreased Bax expression levels(P＜0.05).The intervention effect of Buyang Huanwu Tang combined with EPCs was more significant.Conclusion Buyang Huanwu Tang can inhibit oxidative stress,reduce apoptosis,promote the proliferation and differentiation of EPCs,reduce the area of cerebral infarction,and has a synergistic effect in reducing cerebral ischemia-reperfusion injury when combined with EPCs.
万方数据
维普
