Mechanism of Qingdai in treating eczema based on network pharmacology and molecular docking
Objective To explore the mechanism of external treatment of eczema with Qingdai based on network pharmacology and molecular docking technology.Methods TCMSP database platform was used to search for the effective active ingredients and target genes of Qingdai formula.Gene data related to eczema were searched in GeneCards,TTD,and OMIM databases.The intersection of traditional Chinese medicine targets and disease targets was obtained to find common targets.Protein interaction analysis was performed on the intersection targets based on the String database,and visualization was carried out by importing them into Cytoscape software;David database was used to search the intersecting targets by GO and KEGG enrichment analysis Use AutoDockTools for molecular docking of key active ingredients and core targets.Results After screening,9 main active ingredients of Qingdai were obtained,among which the key ingredients were Indirubin and Beta-sitosterol;There are 37 common targets fordrug diseases,with key core targets including TNF、PTGS2、PPARG、CASP3.A total of 317 GO enriched cells and 113 KEGG enriched cells were obtained,with related signaling pathways including IL-17 signaling,AGE-RAGE signaling,TNF signaling and Th17 cell differentiation;Molecular docking suggests that key active ingredients have good binding affinity with the target.Conclusion Topical application of Qingdai can effectively inhibit immune inflammatory reactions through multiple active ingredients and pathways,achieving the goal of treating eczema.
万方数据
维普
