Preparation of samples for electron microscopy by high osmotic pressure method
AIM:To compare different methods for preparation of samples for electron microscopic observation of Shigellaflexneri surface structures.METHODS:Three Shigella flexneri strains were cultured and used to prepare samples for electron microscopy.Samples were prepared in different osmotic environments.The obtained electron micrographs were compared to obtain the optimal sample preparation conditions.RESULTS:Hyperosmotic environment in sample preparation often makes cytoplasmic shrinkage occur,which is beneficial for cell surface ultrastructure observation.By comparing the electron microscopic pictures of the three strains of Shigellaflexneri,we found that the abundance of bacterial lipopolysaccharides (LPS) was less at 37 ℃ than that at 30 ℃.CONCLUSION:High osmotic pressure sample preparation is better than common methods in observing bacterial cell surface ultrastructures.Using this sample preparation method,electron microscopy analysis revealed that the relative density of LPS of Shigella flexneri varied among different culture temperatures.
Biological electron microscopyShigella flexneriLipopolysaccharides
NETL
NSTL
万方数据
维普
