Objective To evaluate the usability of an individualized symptom network application for human im-munodeficiency virus(HIV)-positive patients.Method In December 2023 to February 2024,the study selected ac-quired immunodeficiency syndrome(AIDS)-infected individuals,case managers,and other patients at the AIDS vol-untary counseling and testing clinic of Shanghai Public Health Clinical Center.Based on the concept of"human-cen-tered design",a series of tests were successively conducted,including brief formative usability test,comprehensive formative usability test,and summative usability test,and quantitative and qualitative data were collected.Result A total of 22 participants were included in the study,and a three-phase usability assessment was conducted,and 4 main themes were formed after coding and classifying the collected qualitative data,and some problems found in the evalu-ation process were corrected in time.Conclusion The function of the program generally meets the requirements of the designers,fulfilled users'expectations,and did not exhibit significant usability problem.This study provides a foun-dation for subsequent program improvements,personalized patient symptom management program design,and clini-cal promotion.