Study on Optimization of Extraction Process and Antioxidation of Water extracts from Prunus Mume
In the article,the extraction process of water extract from Prunus mume was optimized by single factor test and response surface method with Prunus mume as raw material and polysaccha-ride extraction rate as response value.The polysaccharide content of Prunus mume in different produc-ing areas and different flowering periods were determined,and the antioxidant activity of Prunus mume in different producing areas and different flowering periods were evaluated in vitro.The results showed that the optimized extraction process of the water extracts of Prunus mume was as follow:extraction time of 1 h,extraction temperature of 80 ℃,extraction number of 3 times,material-liquid rate of 1:20(g/mL).The content of polysaccharide from Prunus mume was 11.13%under this condition,and the content of polysaccharide was closely related to its area and flowering stage.The polysaccha-ride content in the blooming stage was highest,followed by the initial opening period,and the lowest in the bud period.The in vitro antioxidant activity showed that the water extracts of Prunus mume had strong antioxidant activity,and the origin and flowering stage both influenced its antioxidant activity.In general,the antioxidant activity of the flower bud stage was the strongest and significantly stronger than that of the blooming stage;there was no significant pattern among different areas.