Determination of Glibenclamide Concentration in Placental Perfusion Medium by High Performance Liquid Chromatography
Objective To establish a high performance liquid chromatography (HPLC) method for determining the concentration of glibenclamide in placental perfusion medium. Methods The liquid chromatography column was Symmetry Shield RP C18 (150 mm×4.6 mm, 5 μm) at 40 ℃ . The mobile phase consisted of a mixture of NaH2PO4 (25 mmol/L, pH 5.2)-acetonitrile (1 : 1, V/V). The flow rate was 1.0 mL/min. UV detection wavelength was set at 228 nm. A liquid-liquid extraction pretreatment method was used to detect the concentration of glibenclamide placental perfusion medium. Results A linearity was obtained from 2.0 to 25.0 μg/mL for glibenclamide. The calibration equation of glibenclamide was y=0.226x+0.002, r=0.999 9 (n=6). The relative standard deviations within day and between days were less than 3.1% and less than 9.5% for glibenclamide. The mean recoveries of glibenclamide were 95.32% to 103.35%. Conclusion This method can be used for determining glibenclamide concentration in placental perfusion medium.
GlibenclamideHigh performance liquid chromatographyPlacental perfusion mediumDetectionFoundation item: Applied Basic Research on Projects in Yunnan Province (2008ZC128M)
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维普
