Metal-Organic Frameworks(MOFs)has attracted widespread attention as a new type of porous crystal material.The aim of this study is to investigate its fluorescence quenching-recovery performance as a biosensor.ZIF-8,MIL-101(Fe),MIL-101(Cr),UIO-66,and 2D-pMOFs were synthesized by solvothermal method,and then incubated them with fluorescent probes and complementary DN A to study their fluorescence intensity changes.ZIF-8,MIL-101(Fe)and MIL-101(Cr)had almost no fluorescence quenching-recovery activity,2D-pMOFs had certain fluorescence quenching-recovery efficiency,and UIO-66 had the strongest fluorescence quenching-recovery efficiency.The fluorescence quenching properties of UIO-66 were derived from the photoinduced electron transfer(PET)effect of zirconium metal ions.The fluorescence recovery ability comed from its structural porosity.UIO-66 realized fluorescence quenching/recovery of DNA probes through adsorption/desorption of DNA,and showed great application potential in the field of biosensor.
维普
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