从柚子落果中提取柚皮苷的杂质谱研究

Impurities Spectra of Naringin Products from the Abscission Fruits of Citrus Maxima(Burm.)Merr

张丽 ¹唐官美 ¹韦媛 ¹卢振鸿 ¹韩方方 ¹张雪晴 ¹郭志勇¹

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作者信息

1. 三峡大学生物与制药学院,湖北宜昌 443002;三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌 443002
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摘要

柚皮苷是一种具有广泛用途的二氢黄酮类化合物,为建立基于碱提酸沉柚皮苷提取工艺的质量标准提供物质基础.通过反复重结晶,正向柱层析,制备液相及半制备液相等方法分离纯化,经NMR,圆二色谱等手段鉴定结构.从微量杂质中分离鉴定 12 个杂质,分别为佛手酚(1)、7-Hydroxy-1-benzopiran-2-one(2)、Marmin(3)、Protocatechuic acid(4)、8-(6,7-dihydroxy-3,7-dimethyloct-1-en-3-yl)-5-hydroxy-6,7-furocoumarin(5)、Obacunone(6)、柚皮素(7)、7,4'-Dihydroxyfla-vone(8)、Rhoifolin(9)、Vicenin(10)、Neoeriocitrin(11)和 2S-Neoeriocitrin(12).化合物 5 为首次从柚的果实中分离得到,为建立碱提酸沉工艺提取柚皮苷的质量标准建立提供物质基础.

Abstract

Naringin is a kind of flavonoid with multiform usages.The impurities were isolated and identified from the alkali extraction and acid precipitation process of naringin.Repeated recrystallization,column chromatography,preparative and semipreparative HPLC was employed for isolation and purification.Then the purified impurities were identified by NMR and CD spectra.The twelve impurities were obtained and determined as Bergaptol(1),7-Hydroxy-1-benzopiran-2-one(2),Marmin(3),Protocatechuic acid(4),8-(6,7-dihydroxy-3,7-dimethyloct-1-en-3-yl)-5-hydroxy-6,7-furocoumarin(5),Obacunone(6),Naringin(7),7,4'-Dihydroxyflavone(8),Rhoifolin(9),Vicenin(10),Neoeriocitrin(11)and 2S-Neoeriocitrin(12).The above twelve impurities will help to establish quality control standards of naringin based on alkali extraction and acid precipitation process.

关键词

柚皮苷/碱提酸沉提取工艺/香豆素/黄酮类化合物/杂质

Key words

naringin/alkali extraction and acid precipitation process/coumarins/flavonoids/impurities

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出版年

2025

化学试剂

中国分析测试协会,国药集团化学试剂有限公司,北京国化精试咨询有限公司

化学试剂

影响因子：0.295

ISSN：0258-3283

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