Determination of 9 Aminoglycosides Antibiotic in Pork by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
The method was developed for the determination of trace aminoglycoside antibiotics(AGs)(Amikacin,Apramycin,Hygromycin B,Kanamycin,Paromomycin,Ribostamycin,Spectinomycin,Streptomycin,Tobramycin)residues in pork sample.The pork samples were freeze-dried and ground,then ultrasonically extracted by adding 150 mmol/L EDTA solution and 5％TCA solution,cleaned up by an Oasis HLB column,and then analyzed by ultra-performance liquid chromatography-tandem mass spectrometry.The linear relationships of the 9 AGs were in a good range of 0.01～5 μg/mL(R2 ≥ 0.999).The limits of quantitation were in the range of 0.01～0.29 μg/g.At the three low,medium,and high spiking levels,the average spiking recovery rates of AGs in pork samples ranged from 79.91％to 113.20％,with relative standard deviations of 1.26％～12.40％.This method is simple,sensitive,and suitable for detecting of AGs residues in pork.
aminoglycoside antibioticssolid phase extractionultra-performance liquid chromatography-tandem mass spectrometrypork
万方数据
维普
