化学试剂2025,Vol.47Issue(1) :92-96.DOI:10.13822/j.cnki.hxsj.2024.0329

胶体金免疫层析法快速检测动物源食品中的诺氟沙星

Rapid Detection of Norfloxacin in Animal-Derived Foods by Colloidal Gold Immunochromatography

陈文硕 温宇浩 娄婷婷 潘红蕊 李金花
化学试剂2025,Vol.47Issue(1) :92-96.DOI:10.13822/j.cnki.hxsj.2024.0329

胶体金免疫层析法快速检测动物源食品中的诺氟沙星

Rapid Detection of Norfloxacin in Animal-Derived Foods by Colloidal Gold Immunochromatography

陈文硕 1温宇浩 2娄婷婷 3潘红蕊 1李金花2
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作者信息

  • 1. 天津海关工业产品安全技术中心,天津 300457
  • 2. 中国科学院烟台海岸带研究所 海岸带生态环境监测技术与装备山东省工程研究中心 山东省海岸带环境过程重点实验室,山东烟台 264003
  • 3. 天津科技大学生物工程学院,天津 300222
  • 折叠

摘要

胶体金免疫层析法具有高特异性、高灵敏度、高重复性等特点,能够准确地检测出样品中的目标物,在快速检测领域具有广泛应用.本研究将胶体金免疫层析法用于快速检测动物源食品(猪肉、鸡肉、鱼肉、虾肉、鸡蛋)中的诺氟沙星(Norfloxacin,NOR)残留,对方法进行验证,相对准确度为97.0%.方法的检出限为2 μg/kg,检测性能强.在上述5种不同基质样品中,NOR胶体金快速检测方法的灵敏度为99%、特异度为98%、假阳性率为2%、假阴性率为1%.该方法简单快速、灵敏准确,适于复杂基质中NOR的快速精准检测.

Abstract

Colloidal gold immunochromatography has the characteristics of high specificity,high sensitivity,and high repeatability,which can accurately detect the targeted analytes in the samples,and has a wide range of applications in the field of rapid detection.This study used colloidal gold immunochromatography to rapidly detect norfloxacin(NOR)residues in animal-derived foods(pork,chicken,fish,shrimp,and eggs)and validated the method with a relative accuracy of 97.0%.The limit of detection was down to 2 μg/kg,showing high detectability.By testing the above-mentioned five different matrix samples,the NOR colloidal gold rapid detection method attained a sensitivity of 99%,specificity of 98%,false positive rate of 2%,and false negative rate of 1%.This method proved simple,rapid,sensitive,and accurate,and thereby was practically applicable for the rapid,precise detection of NOR in complicated matrices.

关键词

胶体金免疫层析法/诺氟沙星/方法验证/动物源食品/快速检测

Key words

colloidal gold immunochromatography/norfloxacin/method validation/animal-derived foods/rapid detection

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出版年

2025
化学试剂
中国分析测试协会,国药集团化学试剂有限公司,北京国化精试咨询有限公司

化学试剂

影响因子:0.295
ISSN:0258-3283
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