Rapid Detection of Norfloxacin in Animal-Derived Foods by Colloidal Gold Immunochromatography
Colloidal gold immunochromatography has the characteristics of high specificity,high sensitivity,and high repeatability,which can accurately detect the targeted analytes in the samples,and has a wide range of applications in the field of rapid detection.This study used colloidal gold immunochromatography to rapidly detect norfloxacin(NOR)residues in animal-derived foods(pork,chicken,fish,shrimp,and eggs)and validated the method with a relative accuracy of 97.0%.The limit of detection was down to 2 μg/kg,showing high detectability.By testing the above-mentioned five different matrix samples,the NOR colloidal gold rapid detection method attained a sensitivity of 99%,specificity of 98%,false positive rate of 2%,and false negative rate of 1%.This method proved simple,rapid,sensitive,and accurate,and thereby was practically applicable for the rapid,precise detection of NOR in complicated matrices.