Interactive effect of gene INHA and GnRHR on litter size in swine
An asymmetric PCR-SSCP, PCR-SSCP, PCR-RFLP technology was employed to analyze the polymorphisms of gene INHA and CnRHR in Large White swine and Landrace swine, swine from areas of Ningxiang, Taoyuan, Daweizi, Shazilin, Hainan Wuzhishan. It's found that there were two mutations, G262A and A82G in INHA gene exon II, For gene GnRHR at 5'-UTR there was one mutation A→C at 310 bp.Analysis of their interactive effect was conducted. And the interactive effect was extremely significantl in Large White swine (P<0.01), significant in Landrance(P<0.05)between genotype of G262A in INHA and A310C in GnRHR. The biggest effect of interactive genotype was: AGDD, the smallest effcet of interactive genotype was GGEE. It showed that the interaction had less effect on total number of birth, number born alive(P > 0.05), between A852G in INHA and A310C in GnRHR.