Based on Yunyan87 testing material,we examined the influence of 15.0,10.0,5.0 μg/L concentrations of 8%diethyl aminoethyl hexanoate(DA-6)either singularly applied or applied in combination with calcium chloride on germination and seedling quality of flue-cured tobacco seeds.The results highlighted that:compared to the control treated with water,the emergence rate at 7th and 14th day after sowing were increased by 4.50%-10.40%and 2.74%-8.33%respectively,the seedling rate were increased by 3.19%-8.81%in the combined treatment,and in seedling period,seedling height,stem basal width,maximum leaf area were improved in the combined treatment by 5.99%-17.66%,1.29%-5.47%and 21.1%-73.6%respectively;dry matter accumulation of root,stem,leaf,full stand of seeding and root-to-leaf ratio with combined treatment showed a progressive increase by 25.0%-55.0%,16.7%-33.3%,14.1%-33.8%,16.5%-36.9%and 9.1%-17.0%,respectively,the content of leaf chlorophyll(chlorophyll a + chlorophyll b)with combined treatment was augmented by 26.0%-64.9%;the activity of nitrate reductase with combined treatment elevated by 52.1%-95.9%;catalase(CAT)activity,peroxidase(POD)activity,superoxide dismutase(SOD)enzyme activity and malondialdehyde(MDA)content with combined treatment were decreased by 41.5%-59.3%,27.9%-62.9%,30.3%-81.1%and 34.0%-63.7%,respectively.In conclusion,Yunyan87 seeds treated with 10.0 µg/L DA-6 in combination with 10.0 mmol/L calcium chloride showed the best effect.
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