首页|黄连生物碱对TGEV诱导的ST细胞炎症反应的调节作用

黄连生物碱对TGEV诱导的ST细胞炎症反应的调节作用

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
分别用质量浓度为100、50、25 μg/mL的黄连生物碱(ACC)处理猪传染性胃肠炎病毒(TGEV)感染的猪睾丸细胞(ST),采用噻唑蓝(MTT)法检测细胞的活力;采用硝酸还原酶法检测 NO 含量;采用比色法检测总一氧化氮合成酶(TNOS)和诱导型一氧化氮合成酶(iNOS)活性;采用荧光定量PCR检测TNF-α、IL-1β、IL-6、IL-10等炎症基因的表达.结果表明:100、50 μg/mL 的ACC可极显著提高TGEV感染的ST细胞的存活率,25 μg/mL 的ACC可显著提高TGEV感染的ST细胞存活率;TGEV感染后ST细胞NO含量急剧升高,100、50 μg/mL的ACC可极显著降低ST细胞TNOS和iNOS活性,25 μg/mL ACC可显著降低TNOS和iNOS活性;ACC可极显著下调ST细胞TNF-α、IL-1β、IL-6、IL-10 mRNA的表达水平.表明黄连生物碱可以降低TGEV诱导的ST细胞的炎症反应,缓解病毒对细胞的损伤.
The inflammatory response regulating role of alkaloids of Coptis chinensis in swine testis cells induced by TGEV
The swine testicular(ST)cells infected with transmissible gastroenteritis virus(TGEV)were treated with alkaloids of Coptis chinensis(ACC)with mass concentrations of 100,50 and 25 μg/mL respectively.MTT assay was used to detect the cell viability,the griess method and colorimetric method were used to detect the release of NO,TNOS and iNOS,and Real-time PCR method was used to detect expression changes of inflammatory genes such as TNF-α,IL-1β,IL-6 and IL-10.The results indicated that ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly increase the survival rate of ST cells,and 25 μg/mL ACC could significantly increase the survival rate of ST cells.The content of NO increased sharply after TGEV infection,and the ACC at the mass concentration of 100 and 50 μg/mL could extremely significantly decrease the activities of TNOS and iNOS,and 25 μg/mL ACC could significantly decrease the activities of enzymes TNOS and iNOS.Meanwhile,ACC could extremely significantly down-regulate the expression levels of TNF-α,IL-1β,IL-6 and IL-10 mRNA.We can conclude that the alkaloids of Coptis chinensis could reduce the inflammatory response induced by TGEV and alleviate the damage of virus to cells.

alkaloids of Coptis chinensistransmissible gastroenteritis virusswine testis cellsinflammation-related genes

唐红梅、朱买勋、邓小龙、徐登峰

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重庆市畜牧科学院,重庆 402460

国家生猪技术创新中心,重庆 402460

彭水苗族土家族自治县畜牧发展中心,重庆 409600

黄连生物碱 猪传染性胃肠炎病毒 猪睾丸细胞 炎症相关基因

重庆市自然科学基金面上项目国家重点研发计划项目国家生猪技术创新中心先导项目

cstc2020jcyjmsxmX07572022YFD1601403NCTIP-XD/B12

2024

10.13331/j.cnki.jhau.2024.01.009

湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCD北大核心
影响因子：0.868
ISSN：1007-1032
年,卷(期)：2024.50(1)
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