摘要
为了探明岭南地区特色中药三桠苦的叶绿体基因组结构和序列特征,以三桠苦叶片为材料,采用试剂盒法提取DNA和构建测序文库,利用Novaseq平台进行高通量测序,再结合生物信息学手段进行序列拼接、注释和特征分析.结果显示:三桠苦叶绿体基因组为 158 992 bp的环状四分体分子,含有 134个基因;在三桠苦叶绿体基因组内找到 86个简单重复序列,主要是A/T单核苷酸重复;检测到 26 907个密码子,以A/T结尾的密码子有着更高的使用频次;序列比较分析表明,三桠苦与同科植物在非编码区存在更明显的序列变异;系统进化树揭示不同来源的三桠苦亲缘关系最近,但在基因组成和碱基序列上呈现出多样性.
Abstract
To illustrate the structure and sequence characteristics of chloroplast genome from Melicope pteleifolia,the leaf of M.pteleifolia was used for DNA isolation and library construction.The high-throughput sequencing was fulfilled by Novaseq platform,and then acquired sequence was assembled,annotated and analyzed using bioinformatics means.The results revealed that the chloroplast genome of M.pteleifolia was a circular quadripartite molecule with a size of 158 992 bp and encoded 134 genes.Altogether 86 simple sequence repeat was found in the plastome and the A/T mononucleotide was predominate type.Total 26 907 codon was screened out and those ended with A/T were frequently used.Sequence comparison demonstrated more divergence and occurred in non-coding region between M.pteleifolia and other Rutaceae species.Phylogenetic tree indicated various chloroplast genome sequences of M.pteleifolia shared close relationship and exhibited diversity in gene composition and base sequence.
基金项目
广东省基础与应用基础研究基金(2018A030310116)
广东省大学生创新创业训练计划(S202310571113)
湛江市科技计划(2019A01008)
广东医科大学大学生创新创业训练计划(GDMU2022113)
国家科技期刊平台
NSTL
万方数据