首页|青钱柳叶片甜味性状差异的转录组比较分析

青钱柳叶片甜味性状差异的转录组比较分析

Comparative transcriptome analysis of sweetness traits difference of Cyclocarya paliurus

扫码查看
以湖南武冈种植的甜型和非甜型青钱柳叶为材料,分离其RNA并构建cDNA文库,开展 2 种类型青钱柳的转录组测定.利用转录组数据结合萜类代谢组及其代谢途径分析,研究青钱柳发育过程中甜味性状形成的分子差异.对甜型与非甜型青钱柳叶片转录组的差异基因GO及KEGG分析,共鉴定出 13 个参与三萜类化合物形成的关键酶基因,在 2 种青钱柳叶中具有显著差异性表达模式.结合萜类代谢检测结果,甜型青钱柳叶中 4 类甜味三萜类化合物的积累量显著高于非甜型青钱柳叶的,说明这些基因的差异表达造成了甜味三萜类化合物在叶片中积累量的不同,应是青钱柳甜味差异性状形成的关键原因.
In this study,leaves of sweet and non-sweet taste Cyclocarya paliurus leaves planted in Wugang,Hunan Province were selected for comparative study to illustrate the mechanism of sweet taste formation.The cDNA libraries were constructed and sequenced.The transcriptome date was analyzed with there terpenoid metabolome and the metabolism pathway.The molecular differences in the formation of sweetness traits of Cyclocarya paliurus were explored.Some 13 key enzyme genes involved in triterpene biosynthesis to have significantly different expression patterns between the two materials by GO and KEGG analysis.Combined with terpene metabolism detection,the accumulation of four kinds of sweet triterpenes in sweet Cyclocarya paliurus leaves was significantly higher than that in non-sweet leaves,indicating that the differential expression of these genes maybe causing different accumulation of sweet triterpenoids in leaves,it's key to the formation of sweetness traits in Cyclocarya paliurus.

Cyclocarya paliurustranscriptometriterpenesweetness traits

廖李玲、张学文、赵燕、陆明亮、刘芬、杨华

展开 >

湖南农业大学生物科学技术学院,湖南 长沙 410128

武冈市青钱柳茶叶有限公司,湖南 邵阳 422400

青钱柳 转录组 三萜类化合物 甜味性状

农业部项目湖南硒海生物科技有限公司项目

XCZX-20231412020kjcjs170

2024

湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.868
ISSN:1007-1032
年,卷(期):2024.50(2)
  • 29