拟石莲花属'碧桃'腐烂病病原菌的鉴定及其主要致病物质的测定
Identification of the pathogen causing rot disease on Echeveria cv.'Peach Pride'and determination of the main pathogenic substances
刘爽 1张廷萍 1薛治峰 2刘杨 1王婧 1钱亚新 1秦世雯1
作者信息
- 1. 云南大学资源植物研究院,云南 昆明 650500
- 2. 西北农林科技大学植物保护学院,陕西 杨凌 712100
- 折叠
摘要
采用组织分离法和单孢子纯化法,从拟石莲花属多肉植物品种'碧桃'(Echeveria cv.'Peach Pride')疑似腐烂病的叶片组织获得病原菌,通过形态学和分子序列特征的鉴定以及致病性测定,明确病原菌为尖孢镰刀菌(Fusarium oxysporum).测定尖孢镰刀菌的主要致病物质,发现该病原菌具有较强的细胞壁降解酶活性,并具有一定的产毒素(镰刀菌酸)能力,说明细胞壁降解酶类和毒素是该病原菌侵入、定殖和致病的主要因素.
Abstract
The fungal pathogen was obtained from the leaves of Echeveria cv.'Peach Pride'with suspected rot disease based on tissue separation and single spore separation.According to morphological observation,molecular identification and pathogenicity test,this pathogen was identified as Fusarium oxysporum(F.oxysporum).The main pathogenic substances of F.oxysporum was determined,it was found that the pathogen has strong degrading enzyme activity on cell wall and the ability to produce toxins(fusaric acid),indicating that cel l wall degrading enzymes and toxins are the main factors for the pathogen's invading,colonizing and pathogenesis.
关键词
拟石莲花属多肉植物/腐烂病/尖孢镰刀菌/细胞壁降解酶/镰刀菌酸Key words
Echeveria succulent plant/rot disease/Fusarium oxysporum/cell wall degrading enzyme/fusaric acid引用本文复制引用
基金项目
国家自然科学基金(32060593)
云南省基础研究计划面上项目(202101AT070021)
出版年
2024
国家科技期刊平台
NSTL
万方数据