首页|黔北黑猪骨骼肌中肌纤维类型转化相关circRNAs的筛选

黔北黑猪骨骼肌中肌纤维类型转化相关circRNAs的筛选

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
选取 9 头 6 月龄健康黔北黑猪,随机分成 3 组,采集背最长肌(LDM)和腰大肌(PMM)肌肉组织,通过RNA-seq构建环状RNA(circRNAs)的表达谱,筛选差异表达的circRNAs,在黔北黑猪 2种肌肉中共鉴定出 63个差异表达circRNAs,其中 31个在LDM中表达上调,32个表达下调;对差异表达circRNAs的源基因进行GO、KEGG 富集分析,结果表明,差异表达的 circRNAs 的源基因主要富集在磷酸酶活性的调控、肌节组织活动、肌球蛋白-轻链-磷酸酶活性的正调控等条目;富集的KEGG通路中与肌纤维类型转化相关的通路包括肌动蛋白细胞骨架的调控、ECM-受体互作、PI3K-Akt信号通路.预测差异表达circRNA的靶miRNAs,并筛选对应的靶mRNAs,构建的circRNA-miRNA-mRNA调控网络显示,circ_0003749-miR-27a-MSTN、circ_0003749-miR-27a-PPARγ、circ_0012316-miR-23a-Myh1/2/4、circ_0007093-miR-214-3p-MEF2C、circ_0010118-miR-132-FoxO1和circ_0010118-miR-374a-5p-FoxO1可能通过ceRNA网络调控肌纤维类型的转化.
Screening of circRNAs related to muscle fiber type conversion in skeletal muscle of Qianbei black pigs
Nine 6-month-old healthy Qianbei black pigs were randomly divided into 3 groups.The longissimus dorsi muscle(LDM)and psoas major muscle(PMM)were collected.The expression profiles of circRNAs were constructed by RNA-seq,and the differentially expressed circRNAs in LDM vs.PMM were screened.A total of 63 differentially expressed circRNAs were identified in the two muscles of Qianbei black pigs,of which 31 were up-regulated and 32 were down-regulated in LDM.GO enrichment and KEGG analysis were performed on the source genes of differentially expressed circRNAs.The results showed that the source genes of differentially expressed circRNAs were mainly concentrated in GO items such as the regulation of phosphatase activity,sarcomere activity,and positive regulation of myosin-light-chain-phosphatase activity.In the enriched KEGG pathway,the pathways involving muscle fiber type conversion include the regulation of actin cytoskeleton,ECM-receptor interaction,PI3K-Akt signaling pathway.Finally,target miRNAs of differentially expressed circRNAs were predicted,and corresponding target mRNAs of miRNAs were screened to construct the circRNA-miRNA-mRNA regulatory network.The results showed that circ_0003749-miR-27a-MSTN,circ_0003749-miR-27a-PPARγ,circ_0012316-miR-23a-Myh1/2/4,circ_0007093-miR-214-3p-MEF2C,circ_0010118-miR-132-FoxO1 and circ_0010118-miR-374a-5p-FoxO1 may regulate muscle fiber type conversion through ceRNA network.

Qianbei black pigsskeletal musclemuscle fiber type conversioncircRNA-miRNA-mRNA network

赵园园、孟金柱、宋兴超、安清明、吴震洋

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铜仁学院农林工程与规划学院,贵州 铜仁 554300

湖南农业大学动物医学院,湖南 长沙 410128

黔北黑猪 骨骼肌 肌纤维类型转化 circRNA-miRNA-mRNA网络

贵州省教育厅青年科技人才成长项目贵州省高层次创新型人才培养项目(千层次)贵州省科技厅重点实验室项目铜仁市科技局科技支撑计划

黔教合KY字[2020]1662022-2020-044黔科合平台人才[2020]2003号铜市科研[2020]128号

2024

10.13331/j.cnki.jhau.2024.02.011

湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCD北大核心
影响因子：0.868
ISSN：1007-1032
年,卷(期)：2024.50(2)
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