云南金荞麦叶斑病病原菌的鉴定及其生物学特性
Identification and biological characterization of the pathogen causing leaf spot of Fagopyrum dibotrys in Yunnan
卢文洁 1尹桂芳 1隆文杰 1李程鹏 1孙道旺 1王莉花1
作者信息
- 1. 云南省农业科学院生物技术与种质资源研究所,云南 昆明 650205;云南省农业生物技术重点实验室,云南昆明 650205;农业农村部西南作物基因资源与种质创制重点实验室,云南 昆明 650205
- 折叠
摘要
从云南省嵩明县试验基地采集金荞麦叶斑病病叶,采用组织分离法分离培养病原菌,根据柯赫氏法则测定病原菌的致病性,观察病原菌形态学特征,并与 ITS、TUB2 系统发育分析相结合鉴定病原菌,测定病原菌的生物学特性.结果表明:云南金荞麦叶斑病病原菌代表菌株 GY3 的孢子形态与拟盘多毛孢属(Pestalotiopsis)真菌相似,菌株GY3的rDNA-ITS及TUB2 联合序列与小孢拟盘多毛孢(P.microspora)2 个菌株(ZK1-1、ZK5-5)的序列聚在同一分支,鉴定认为金荞麦叶斑病的病原菌为小孢拟盘多毛孢(P.microspora);病原菌生物学特性测定结果表明,GY3 菌丝生长最适培养基为 PDA,以 PDA 培养基培养 GY3,菌丝生长最适 pH 为 7,最适温度为25℃,菌丝的致死温度为50℃(水浴10 min),连续光照最有利于菌丝生长,菌丝生长的最适的碳源和氮源分别为葡萄糖和蛋白胨.
Abstract
Samples of leaf spot of Fagopyrum dibotrys(F.dibotrys)were collected from experimental base of Songming,Yunnan province,and the pathogen was isolated and cultured by tissue separation method.The pathogenicity of the pathogen was tested according to Koch's rule.The pathogen was identified through morphological characteristics and phylogenetic analysis of ITS and TUB2 genes,and the biological characteristics of the pathogen was investigated.The results showed that the spore morphology of strain GY3,a representitive of the pathogen causing F.dibotrys leaf spot,was similar to the fungi of Pestalotiopsis.The rDNA-ITS and TUB2 sequences of strain GY3 was belonged to the branch which the rDNA-ITS and TUB2 sequences of two isolates(ZK1-1,ZK5-5)of Pestalotiopsis Microspora(P.microspora)belonged to,thus the pathogen causing leaf spot of F.dibotrys was identified as P.microspora.The optimum media for mycelia growth of strain GY3 was PDA.On PDA media,the optimum pH value for mycelia growth of strain GY3 was pH7,the optimum temperature for mycelia growth was 25℃and the fatal temperature for mycelia was 50℃for 10 min.Continuous illumination was favorable for mycelia growth and the optimum carbon source and nitrogen source for mycelia growth were glucose and peptone,respectively.
关键词
金荞麦/叶斑病/病原菌鉴定/小孢拟盘多毛孢/生物学特性/云南Key words
Fagopyrum dibotrys/leaf spot/pathogen identification/Pestalotiopsis microspora/biological characteristics/Yunnan引用本文复制引用
基金项目
云南省农业基础研究联合专项(202101BD070001-122)
财政部和农业农村部-国家现代农业产业技术体系(CARS-07-C-2)
云南省重大科技专项与重点研发计划(202202AE090020)
出版年
2024
国家科技期刊平台
NSTL
万方数据