Anti-damage effects of goat rumen epithelial cells induced by lipopolysaccharide
Goat rumen epithelial cells(GRECs)were cultured in medium containing 1 μg/mL of LPS for 3 h,6 h and 9 h to detect cell viability,antioxidant indices,genes expression of inflammatory factors and tight junction protein.The results showed as follows:1)The viability of GRECs in the group treated with LPS for 3 h was significantly lower than that in groups treated with LPS for 6 h and 9 h,but there was no significant difference in GRECs activity between groups treated with LPS for 6 h and 9 h.2)The glutathione peroxidase(GSH-PX)activity of GRECs treated with LPS for 3 h was extremely significantly lower than that in cells treated with LPS for 6 h and 9 h(P<0.01),whereas the malondialdehyde(MDA)content,on the contrary,was extremely significantly higher in cells treated with LPS for 3 h.The superoxide dismutase(SOD)and catalase(CAT)activities of GRECs significantly increased and the reactive oxygen(ROS)concentration extremely decreased with the prolongation of LPS treatment time.3)The relative expression levels of TNF-ɑ and IL-1β in GRECs treated with LPS for 3 h were significantly higher than that in cells treated with LPS for 6 h and 9 h.The relative expression of IL-1β was significantly decreased with the extension of LPS treatment time.The relative expression levels of IL-6 had no significant difference among all group.4)The distribution of ZO-1 was low in GRECs treated with LPS for 3 h,but increased with the extension of LPS treatment time.The relative expression levels of Claudin-1 in GRECs treated with LPS for 3 h were significantly higher than that in cells treated with LPS for 6 h and 9 h.The results showed that the GRECs could alleviate oxidative damage by improving their own antioxidant and anti-inflammatory capacities with the extension of LPS treatment time,then improve barrier function of the cells.
lipopolysaccharidegoat rumen epithelial cellsoxidation resistanceanti-inflammatory effecttight junction protein
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