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基于全基因组关联分析的水稻穗长QTL定位及其候选基因分析

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分别于 2021年和 2022 年在湖南长沙种植 254份水稻 3K种质资源,在成熟期测定各种质的穗长.结合种质基因型进行全基因组关联分析,共检测到 3个穗长QTL,分布于水稻第 1、5、6号染色体,分别命名为qPL-1、qPL-5和qPL-6,相对贡献率为 9.06%~28.27%;结合QTL区间内基因功能注释和基因不同单倍型的穗长差异显著性分析结果,最终获得qPL-1、qPL-5和qPL-6的候选基因,其中qPL-6与sped1-D共定位,Os01g0715600、Os05g0132700为调控穗长的新候选基因;对Os01g0715600、Os05g0132700 和Os06g0597500的单倍型进行聚合分析,发现这 3个基因存在累加效应.
Panicle length QTL mapping and candidate gene analysis based on genome-wide association analysis in rice
In this study,254 rice 3K germplasm resources were used as research materials,and they were planted in Changsha,Hunan Province,in 2021 and 2022,respectively.The panicle lengths of the various germplasm were measured at heading stage.Combined with germplasm genotypes for genome-wide association study,three panicle length QTL were detected on rice chromosomes 1,5,and 6,named qPL-1,qPL-5,and qPL-6,respectively.These QTL explained phenotypic variation range from 9.06%to 28.27%of the variation of spike length.The candidate genes of qPL-1,qPL-5 and qPL-6 were finally obtained by combining the gene function annotation in the QTL interval and the significant difference of the panicle length of different haplotypes.With the co-localization of qPL-6 and sped1-D,Os01g0715600 and Os05g0132700 were identified as the new candidate genes for the regulation of spike length.Polymerization analysis of the elite haplotypes of Os01g0715600,Os05g0132700 and Os06g0597500 revealed a superposition effect within the three genes.

ricepanicle lengthQTL(quantitative trait loci)candidate genehaplotypegene pyramiding

祝小雅、闫蕴韬、桂金鑫、石居斌、张海清、贺记外

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湖南农业大学农学院,湖南 长沙 410128

水稻 穗长 数量性状位点 候选基因 单倍型 基因聚合

2024

湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.868
ISSN:1007-1032
年,卷(期):2024.50(4)