摘要
运用超高效液相色谱-串联质谱联用技术,从烤烟品种 G80 不同成熟期(打顶当天及打顶后第 10、20、30 天)烟叶中鉴定出亲水性代谢物 211 种、亲脂性代谢物 74 种;共筛选出 94 个差异代谢物,归属于氨基酸、糖及糖苷类、脂类、胺类、生物碱、苯丙酸及其衍生物、萜类、核苷酸和其他类等 9大类,其中L-苯丙氨酸、DL-苯丙氨酸、DL-酪氨酸、蔗糖和甘油酸为G80烟叶成熟过程中的关键代谢物.代谢通路分析发现,G80不同成熟期烟叶差异代谢物均显著富集于苯丙氨酸代谢途径,其代谢产物木脂素、苯丙酸、黄酮类和香豆素及其衍生物等在成熟后期大量积累,有助于增强烟叶的抗氧化能力和促进香气的形成.
Abstract
Two hundred and eleven hydrophilic metabolites and 74 lipophilic metabolites from tobacco leaves of flue-cured tobacco variety G80 at different maturity stages(on the day of topping and on the 10th,20th and 30th days of topping)were identified using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).A total of 94 differential metabolites were screened out,including nine major groups:amino acids,sugars and glycosides,lipids,amines,alkaloids,phenylalanine and its derivatives,terpenes,nucleotides,and others.The key metabolites in tobacco leaves during the maturation process of flue-cured tobacco variety G80 were found to be L-phenylalanine,DL-phenylalanine,DL-tyrosine,sucrose and glyceric acid.Metabolic pathway analysis showed that the differential metabolites of tobacco leaves in flue-cured tobacco variety G80 at different maturation stages were significantly enriched in the phenylalanine metabolic pathway.Metabolites such as lignans,phenylpropanoic acid,flavonoids,and coumarins and their derivatives,which were accumulated in large quantities during the late stage of maturation,could improve the antioxidant capacity and aroma formation of the tobacco leaves.
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