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生菜HSF基因家族的全基因组鉴定与表达分析

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利用生菜的全基因组数据,采用生物信息学方法,从生菜中鉴定出了 35个LsHSF基因,按照其在染色体上的分布顺序命名为LsHSF1,LsHSF2,…,LsHSF35,并对其理化性质、染色体定位、与拟南芥的进化关系、蛋白保守基序、组织表达水平及褪黑素处理后高温条件下的表达量进行分析.亚细胞定位预测分析结果显示,除LsHSF11、LsHSF17定位在叶绿体,LsHSF34定位在液泡外,其余基因都定位在细胞核中;35 个LsHSF基因不均等地分布在 9条染色体上;蛋白保守基序预测分析结果显示,所有LsHSF基因都含有Motif 1 与Motif 2,且存在紧密联系;系统发育树分析结果表明,HSF蛋白分为A、B和C共 3组,被划分为 14个主要亚家族;组织表达水平分析结果表明,HSF等基因具有特异性表达模式;高温处理能够刺激HSF基因家族成员的表达.
Genome-wide identification and expression analysis of HSF gene family in lettuce
Using the whole-genome data of lettuce,35 LsHSF genes were identified from lettuce through bioinformatics methods,and named LsHSF1,LsHSF2,…,LsHSF35 according to the distribution order on their chromosome.Their physicochemical properties,chromosomal localization,evolutionary relationship with Arabidopsis,protein conserved motifs,tissue expression levels,and expression levels under high-temperature conditions after melatonin treatment were further analyzed in this study.The results showed that subcellular localization prediction analysis indicated that,except for LsHSF11 and LsHSF17 located in chloroplasts,and LsHSF34 located outside the vacuole,the rest of the genes were located in the nucleus.The 35 LsHSF genes were unevenly distributed on 9 chromosomes.Protein conserved motif prediction analysis revealed that all LsHSF genes contained two closely related Motif 1 and Motif 2.From phylogenetic analysis,HSF proteins were divided into three groups:A,B,and C and could be further subdivided into 14 major subfamilies.Tissue expression level analysis results indicated that HSF genes exhibited specific expression patterns.High-temperature treatment could stimulate the expression of HSF gene family members.

lettuceLsHSF genegenome-widehigh-temperature stress

陈丽、范双喜

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湖南省蔬菜研究所,湖南 长沙 410125

北京农业职业学院园艺园林学院,北京 102400

生菜 LsHSF基因 全基因组 高温胁迫

2024

湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.868
ISSN:1007-1032
年,卷(期):2024.50(4)