摘要
以大豆卵磷脂为乳化剂,菜籽油为油相,制备一种稳定纳米乳液体系包埋益智酮乙.通过单因素试验、响应面试验优化纳米乳液制备条件,运用纳米粒度仪、激光共聚焦显微镜和傅里叶红外光谱仪等测量乳液的粒径、多分散系数(PDI)、Zeta 电位、包封率、稳定性、光谱特性等.结果表明:在最佳制备工艺参数(大豆卵磷脂质量浓度 18.4 g/L、均质压强 172.35 MPa、均质次数 7次和益智酮乙质量浓度 7 mg/mL)下,益智酮乙纳米乳液平均粒径为(175.37±4.33)nm,PDI为0.13±0.02,包封率为(91.00±0.03)%;益智酮乙包埋量不影响乳液的粒径和PDI,包埋 7 mg/mL益智酮乙的纳米乳液包封率最高;益智酮乙纳米乳液的微观结构为球状,乳液颗粒分布均匀,于 4℃贮藏 28 d时,乳液的粒径、PDI和包封率均无明显变化,乳液系统稳定,添加NaCl浓度≤30 mmol/L时,乳液能维持较为稳定的状态,NaCl浓度≥50 mmol/L时,随着NaCl浓度的升高,乳液的粒径和PDI显著升高,Zeta电位绝对值显著降低,乳液稳定性下降;菜籽油、大豆卵磷脂和益智酮乙之间没有形成共价键,也未发生化学相互作用.
Abstract
Using soybean lecithin as an emulsifier and rapeseed oil as the oil phase,a stable nanoemulsion system encapsulating yakuchinone B was prepared.The preparation conditions for the nanoemulsion were optimized through single-factor experiments and response surface methodology.Measurement of the emulsion was performed using a nanoparticle size analyzer,a laser confocal microscope,and a Fourier transform infrared spectrometer to assess the particle size,polydispersity index(PDI),Zeta potential,encapsulation efficiency,stability,and spectral characteristics.The results indicated that under the optimal preparation process parameters(soybean lecithin mass concentration of 18.4 g/L,homogenization pressure of 172.35 MPa,homogenization cycles of 7,and yakuchinone B mass concentration of 7 mg/mL),the average particle size of the yakuchinone B nanoemulsion was(175.37±4.33)nm,the PDI was 0.13±0.02,and the encapsulation efficiency was(91.00±0.03)％.The encapsulation amount of yakuchinone B did not affect the particle size and PDI of the emulsion,and the nanoemulsion with 7 mg/mL of yakuchinone B had the highest encapsulation efficiency.The microstructure of the yakuchinone B nanoemulsion was spherical and uniformly distributed.When stored at 4 ℃ for 28 days,there were no significant changes in the particle size,PDI,and encapsulation efficiency of the emulsion,demonstrating the system's stability.When the concentration of added NaCl was≤30 mmol/L,the emulsion could maintain a relatively stable state.When the NaCl concentration was≥50 mmol/L,the particle size and PDI of the emulsion increased significantly,and the absolute value of the Zeta potential decreased significantly,leading to a decrease in emulsion stability.There were no covalent bonds formed between rapeseed oil,soybean lecithin,and huperzine B,and no chemical interactions occurred.
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