首页|"湘芯一号"肉鸡60K液相育种芯片的设计及基因组预测效果

"湘芯一号"肉鸡60K液相育种芯片的设计及基因组预测效果

扫码查看
利用基因组重测序技术对湘佳黑鸡、湘佳白羽肉鸡、湘佳黄鸡 2 号、湘佳黑鸡 2 号、邵伯鸡群体进行基因型分型和遗传结构分析,筛选出高质量的单核苷酸多态性(SNP)位点.基于90日龄体质量(BW90)和70~90日龄剩余采食量(RFI)性状的方差组分和育种值估计,发现选择"京芯一号"10K位点子集估计的育种值准确性在 0.915 以上.利用QTLdb数据库进行功能位点注释,筛选出 2 851 个与肉鸡经济性状相关的重要SNP位点.最终得到 60 784 个高质量、高多态性的SNP位点,用于"湘芯一号"肉鸡 60K液相育种芯片的设计.检测结果表明,"湘芯一号"肉鸡 60K 液相育种芯片基因型一致性在 95%左右,个体和位点检出率均维持在 99%以上,表明该育种芯片具有良好的基因组预测效果.
Design and genomic prediction effects of the"Xiangxin No.1"broiler 60K liquid phase breeding chip
This study employed genomic resequencing technology to analyze the genotype and the genetic structure of Xiangjia Black Chicken,Xiangjia White Broiler,Xiangjia Yellow Chicken No.2,Xiangjia Black Chicken No.2 and Shaobo Chicken populations,and to identify high-quality single nucleotide polymorphism(SNP)loci.Based on the variance components between 90 day old body weight(BW90)and 70 to 90 day old residual feed intake(RFI)and the breeding value estimations,it was found that the accuracy of breeding value estimations using the"Jingxin No.1"10K SNP loci subset was above 0.915.Functional locus annotation using the QTLdb database led to the selection of 2 851 significant SNP loci associated with economic traits of broilers.Ultimately,60 784 high-quality and highly polymorphic SNP loci were identified for the design of the"Xiangxin No.1"broiler 60K liquid phase breeding chip.The detection results of the chip show that the genotype consistency of the"Xiangxin No.1"broiler 60K liquid phase breeding chip is around 95%,and both individual and locus detection rates are maintained above 99%,indicating that the breeding chip has good genomic prediction effects.

broilerliquid phase chipmolecular markerswhole genome selectionbreeding

贺喜、马豪杰、刘会超、肖健、刘勃攻、欧阳清渊、张海涵

展开 >

湖南农业大学动物科学技术学院,湖南 长沙 410128

湖南省家禽安全生产工程技术研究中心,湖南 长沙 410128

岳麓山实验室,湖南 长沙 410128

湖南湘佳牧业股份有限公司,湖南 常德 415300

展开 >

肉鸡 液相芯片 分子标记 全基因组选择 育种

2024

湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCD北大核心
影响因子:0.868
ISSN:1007-1032
年,卷(期):2024.50(6)