湖南农业大学学报(自然科学版)2024,Vol.50Issue(6) :10-21.DOI:10.13331/j.cnki.jhau.2024.06.002

玉米MADS-box基因家族的鉴定及表达模式分析

Identification of MADS-box gene family in maize and their expression pattern analysis

刘泓畅 马恩慧 王翠玲 张严玲 陈钰姝 刘青青
湖南农业大学学报(自然科学版)2024,Vol.50Issue(6) :10-21.DOI:10.13331/j.cnki.jhau.2024.06.002

玉米MADS-box基因家族的鉴定及表达模式分析

Identification of MADS-box gene family in maize and their expression pattern analysis

刘泓畅 1马恩慧 1王翠玲 1张严玲 1陈钰姝 1刘青青1
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作者信息

  • 1. 河南科技大学农学院,河南 洛阳 471000
  • 折叠

摘要

利用生物信息学方法对玉米(Zea mays)骨干自交系黄早四及玉米自交系 B73 全基因组中的 MADS-box基因家族成员进行鉴定并依据转录组数据分析其表达模式.结果显示:黄早四基因组中有87个MADS-box家族成员,B73 基因组中该家族成员有 84 个;2 个自交系间的 MADS-box 成员共存在 113 个共线基因对,表明MADS-box家族在 2 个自交系间进化较为保守;在花粉粒和双核雄性配子体组织中显著表达的MADS-box家族成员数量最少,穗原基中显著表达的成员数量最多;非生物胁迫下,在冷、热、紫外线逆境胁迫处理下,相比于对照组,MADS-box 家族成员中多为下调表达,而在盐、干旱、干旱及盐复合逆境胁迫下多为上调表达,提示MADS-box家族成员通过不同的调节方式和途径参与玉米生长发育和逆境胁迫响应.

Abstract

In this study,we used bioinformatics method to identify MADS-box family members in the whole genome of maize(Zea mays)backbone inbred line Huangzaosi and maize inbred line B73,and then analyzed their expression patterns based on the transcriptome data.The results showed that 87 members of MADS-box gene family were identified in the genome of Huangzaosi,and 84 members were identified in the genome of B73.113 homologous gene pairs were found among the members of MADS-box gene family between the two inbred lines,indicating the MADS-box gene family were evolutionarily conservative between the two inbred lines.The MADS-box genes expressed the least in microspore and bicellular male gametophyte,while the most in the tassel primordia tissue.Compared to the control,the expression analysis of under abiotic stress showed that the MADS-box genes were almost down-regulated under cold,heat,and UV stress,while most of the MADS-box genes were up-regulated under salt,drought stress or the combined stress of drought and salt.The analysis results of expression pattern suggested that members of MADS-box gene family were involved in the development and the response to abiotic stress in maize through different regulatory ways.

关键词

玉米/黄早四/MADS-box基因家族/全基因组鉴定

Key words

Zea mays/Huangzaosi/MADS-box gene family/genome-wide identification

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出版年

2024
湖南农业大学学报(自然科学版)
湖南农业大学

湖南农业大学学报(自然科学版)

CSTPCDCSCD北大核心
影响因子:0.868
ISSN:1007-1032
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